Recent advances in the application of solution NMR spectroscopy to multi-span integral membrane proteins

Hak Jun Kim, Stanley C. Howell, Wade D. Van Horn, Young Ho Jeon, Charles R. Sanders

Research output: Contribution to journalReview articlepeer-review

131 Scopus citations


Integral membrane proteins (IMPs), which can only be released from the membrane by disruption of the membrane, perform a host of vital cellular functions as receptors, transporters, channels, electrical and photo-transducers. The aggregate molecular weight of an IMP-model membrane complex offers some insight into the challenge that a solution NMR structural effort will present under the most favorable circumstances. Cell-free systems have emerged as a promising alternative for preparing large quantities of isotopically labeled membrane proteins. Higher complexity eukaryotic hosts have also been used for producing protein samples for NMR characterization. These systems include baculovirus-infected insect cells and transfected mammalian cells. Successful NMR studies of IMPs have been carried out in organic solvent mixtures. A report from MacKenzie and co-workers has demonstrated that the addition of very modest amounts of phospholipids to micelles can result in the enhancements of NMR spectral quality for some integral membrane proteins.

Original languageEnglish (US)
Pages (from-to)335-360
Number of pages26
JournalProgress in Nuclear Magnetic Resonance Spectroscopy
Issue number4
StatePublished - Nov 2009
Externally publishedYes


  • Magnetic resonance
  • Membrane protein
  • Micelle
  • Structure determination

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Nuclear and High Energy Physics
  • Spectroscopy


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