Abstract
To understand how molecules function in biological systems, new methods are required to obtain atomic resolution structures from biological material under physiological conditions. Intense femtosecond-duration pulses from X-ray free-electron lasers (XFELs) can outrun most damage processes, vastly increasing the tolerable dose before the specimen is destroyed. This in turn allows structure determination from crystals much smaller and more radiation sensitive than previously considered possible, allowing data collection from room temperature structures and avoiding structural changes due to cooling. Regardless, high-resolution structures obtained from XFEL data mostly use crystals far larger than 1 μm3 in volume, whereas the X-ray beam is often attenuated to protect the detector from damage caused by intense Bragg spots. Here, we describe the 2 Å resolution structure of native nanocrystalline granulovirus occlusion bodies (OBs) that are less than 0.016 μm3 in volume using the full power of the Linac Coherent Light Source (LCLS) and a dose up to 1.3 GGy per crystal. The crystalline shell of granulovirus OBs consists, on average, of about 9,000 unit cells, representing the smallest protein crystals to yield a high-resolution structure by X-ray crystallography to date. The XFEL structure shows little to no evidence of radiation damage and is more complete than a model determined using synchrotron data from recombinantly produced, much larger, cryocooled granulovirus granulin microcrystals. Our measurements suggest that it should be possible, under ideal experimental conditions, to obtain data from protein crystals with only 100 unit cells in volume using currently available XFELs and suggest that single-molecule imaging of individual biomolecules could almost be within reach.
Original language | English (US) |
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Pages (from-to) | 2247-2252 |
Number of pages | 6 |
Journal | Proceedings of the National Academy of Sciences of the United States of America |
Volume | 114 |
Issue number | 9 |
DOIs | |
State | Published - Feb 28 2017 |
Keywords
- Bioimaging
- Nanocrystals
- SFX
- Structural biology
- XFEL
ASJC Scopus subject areas
- General
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Dive into the research topics of 'Atomic structure of granulin determined from native nanocrystalline granulovirus using an X-ray free-electron laser'. Together they form a unique fingerprint.Datasets
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Structure of native granulovirus polyhedrin determined using an X-ray free-electron laser
Gati, C. (Contributor), Oberthuer, D. (Contributor), Yefanov, O. (Contributor), Bunker, R. D. (Contributor), Stellato, F. (Contributor), Chiu, E. (Contributor), Yeh, S. (Contributor), Aquila, A. (Contributor), Basu, S. (Contributor), Bean, R. (Contributor), Beyerlein, K. R. (Contributor), Botha, S. (Contributor), Boutet, S. (Contributor), DePonte, D. P. (Contributor), Doak, R. B. (Contributor), Fromme, R. (Contributor), Galli, L. (Contributor), Grotjohann, I. (Contributor), James, D. R. (Contributor), Kupitz, C. (Contributor), Lomb, L. (Contributor), Messerschmidt, M. (Contributor), Nass, K. (Contributor), Rendek, K. (Contributor), Shoeman, R. L. (Contributor), Wang, D. (Contributor), Weierstall, U. (Contributor), White, T. A. (Contributor), Williams, G. J. (Contributor), Zatsepin, N. A. (Contributor), Fromme, P. (Contributor), Spence, J. C. H. (Contributor), Goldie, K. N. (Contributor), Jehle, J. A. (Contributor), Metcalf, P. (Contributor), Barty, A. (Contributor) & Chapman, H. N. (Contributor), Protein Data Bank (PDB), Feb 22 2017
DOI: 10.2210/pdb5G0Z, https://www.wwpdb.org/pdb?id=pdb_00005g0z
Dataset
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Structure of recombinant granulovirus polyhedrin
Gati, C. (Contributor), Oberthuer, D. (Contributor), Yefanov, O. (Contributor), Bunker, R. D. (Contributor), Stellato, F. (Contributor), Chiu, E. (Contributor), Yeh, S. (Contributor), Aquila, A. (Contributor), Basu, S. (Contributor), Bean, R. (Contributor), Beyerlein, K. R. (Contributor), Botha, S. (Contributor), Boutet, S. (Contributor), DePonte, D. P. (Contributor), Doak, R. B. (Contributor), Fromme, R. (Contributor), Galli, L. (Contributor), Grotjohann, I. (Contributor), James, D. R. (Contributor), Kupitz, C. (Contributor), Lomb, L. (Contributor), Messerschmidt, M. (Contributor), Nass, K. (Contributor), Rendek, K. (Contributor), Shoeman, R. L. (Contributor), Wang, D. (Contributor), Weierstall, U. (Contributor), White, T. A. (Contributor), Williams, G. J. (Contributor), Zatsepin, N. A. (Contributor), Fromme, P. (Contributor), Spence, J. C. H. (Contributor), Goldie, K. N. (Contributor), Jehle, J. A. (Contributor), Metcalf, P. (Contributor), Barty, A. (Contributor) & Chapman, H. N. (Contributor), Protein Data Bank (PDB), Feb 22 2017
DOI: 10.2210/pdb5G3X, https://www.wwpdb.org/pdb?id=pdb_00005g3x
Dataset