Description
Experimental Technique/Method:X-RAY DIFFRACTION
Resolution:1.75
Classification:ELECTRON TRANSPORT
Release Date:2015-08-19
Deposition Date:2015-04-28
Revision Date:2015-09-02#2016-12-14#2017-02-08#2017-11-22#2018-02-14
Molecular Weight:37439.36
Macromolecule Type:Protein
Residue Count:334
Atom Site Count:2626
DOI:10.2210/pdb4ziz/pdb
Abstract:
Serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs) enables high-resolution protein structure determination using micrometre-sized crystals at room temperature with minimal effects from radiation damage. SFX requires a steady supply of microcrystals intersecting the XFEL beam at random orientations. An LCP-SFX method has recently been introduced in which microcrystals of membrane proteins are grown and delivered for SFX data collection inside a gel-like membrane-mimetic matrix, known as lipidic cubic phase (LCP), using a special LCP microextrusion injector. Here, it is demonstrated that LCP can also be used as a suitable carrier medium for microcrystals of soluble proteins, enabling a dramatic reduction in the amount of crystallized protein required for data collection compared with crystals delivered by liquid injectors. High-quality LCP-SFX data sets were collected for two soluble proteins, lysozyme and phycocyanin, using less than 0.1 mg of each protein.
Resolution:1.75
Classification:ELECTRON TRANSPORT
Release Date:2015-08-19
Deposition Date:2015-04-28
Revision Date:2015-09-02#2016-12-14#2017-02-08#2017-11-22#2018-02-14
Molecular Weight:37439.36
Macromolecule Type:Protein
Residue Count:334
Atom Site Count:2626
DOI:10.2210/pdb4ziz/pdb
Abstract:
Serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs) enables high-resolution protein structure determination using micrometre-sized crystals at room temperature with minimal effects from radiation damage. SFX requires a steady supply of microcrystals intersecting the XFEL beam at random orientations. An LCP-SFX method has recently been introduced in which microcrystals of membrane proteins are grown and delivered for SFX data collection inside a gel-like membrane-mimetic matrix, known as lipidic cubic phase (LCP), using a special LCP microextrusion injector. Here, it is demonstrated that LCP can also be used as a suitable carrier medium for microcrystals of soluble proteins, enabling a dramatic reduction in the amount of crystallized protein required for data collection compared with crystals delivered by liquid injectors. High-quality LCP-SFX data sets were collected for two soluble proteins, lysozyme and phycocyanin, using less than 0.1 mg of each protein.
| Date made available | 2015 |
|---|---|
| Publisher | RCSB-PDB |