Visualizing macromolecular complexes with in situ liquid scanning transmission electron microscopy

James E. Evans, Katherine L. Jungjohann, Peony C.K. Wong, Po Lin Chiu, Gavin H. Dutrow, Ilke Arslan, Nigel D. Browning

Research output: Contribution to journalArticlepeer-review

73 Scopus citations


A central focus of biological research is understanding the structure/function relationship of macromolecular protein complexes. Yet conventional transmission electron microscopy techniques are limited to static observations. Here we present the first direct images of purified macromolecular protein complexes using in situ liquid scanning transmission electron microscopy. Our results establish the capability of this technique for visualizing the interface between biology and nanotechnology with high fidelity while also probing the interactions of biomolecules within solution. This method represents an important advancement towards allowing future high-resolution observations of biological processes and conformational dynamics in real-time.

Original languageEnglish (US)
Pages (from-to)1085-1090
Number of pages6
Issue number11
StatePublished - Nov 2012
Externally publishedYes


  • Aberration corrected STEM
  • Cryo-EM
  • In situ TEM
  • Liquid STEM
  • NLP
  • Nanolipoprotein
  • STEM
  • TEM

ASJC Scopus subject areas

  • Structural Biology
  • Cell Biology


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