Veal heart ribonuclease P has an essential RNA component

Eiko Akaboshi; Cecilia Guerrier-Takada; Sidney Altman

doi:10.1016/0006-291X(80)91430-8

Veal heart ribonuclease P has an essential RNA component

Eiko Akaboshi, Cecilia Guerrier-Takada, Sidney Altman

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

The activity of RNase P (EC 3.1.26.5) from veal heart can be abolished by pretreatment of the enzyme preparation with micrococcal nuclease, pancreatic RNase A, or RNase T₁. This indicates that veal heart RNase P contains an RNA component essential for function of the enzyme as has also been shown for E. coli RNase P (1–3). Additionally, veal heart RNase P has a buoyant density in Cs₂SO₄ of 1.33 g/cm³, which is intermediate between that of protein and nucleic acid.

Original language	English (US)
Pages (from-to)	831-837
Number of pages	7
Journal	Topics in Catalysis
Volume	96
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(80)91430-8
State	Published - 1980
Externally published	Yes

ASJC Scopus subject areas

Catalysis
Chemistry(all)
Biochemistry
Biophysics
Molecular Biology

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10.1016/0006-291X(80)91430-8

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title = "Veal heart ribonuclease P has an essential RNA component",

abstract = "The activity of RNase P (EC 3.1.26.5) from veal heart can be abolished by pretreatment of the enzyme preparation with micrococcal nuclease, pancreatic RNase A, or RNase T1. This indicates that veal heart RNase P contains an RNA component essential for function of the enzyme as has also been shown for E. coli RNase P (1–3). Additionally, veal heart RNase P has a buoyant density in Cs2SO4 of 1.33 g/cm3, which is intermediate between that of protein and nucleic acid.",

author = "Eiko Akaboshi and Cecilia Guerrier-Takada and Sidney Altman",

year = "1980",

doi = "10.1016/0006-291X(80)91430-8",

language = "English (US)",

volume = "96",

pages = "831--837",

journal = "Topics in Catalysis",

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T1 - Veal heart ribonuclease P has an essential RNA component

AU - Akaboshi, Eiko

AU - Guerrier-Takada, Cecilia

AU - Altman, Sidney

PY - 1980

Y1 - 1980

N2 - The activity of RNase P (EC 3.1.26.5) from veal heart can be abolished by pretreatment of the enzyme preparation with micrococcal nuclease, pancreatic RNase A, or RNase T1. This indicates that veal heart RNase P contains an RNA component essential for function of the enzyme as has also been shown for E. coli RNase P (1–3). Additionally, veal heart RNase P has a buoyant density in Cs2SO4 of 1.33 g/cm3, which is intermediate between that of protein and nucleic acid.

AB - The activity of RNase P (EC 3.1.26.5) from veal heart can be abolished by pretreatment of the enzyme preparation with micrococcal nuclease, pancreatic RNase A, or RNase T1. This indicates that veal heart RNase P contains an RNA component essential for function of the enzyme as has also been shown for E. coli RNase P (1–3). Additionally, veal heart RNase P has a buoyant density in Cs2SO4 of 1.33 g/cm3, which is intermediate between that of protein and nucleic acid.

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U2 - 10.1016/0006-291X(80)91430-8

DO - 10.1016/0006-291X(80)91430-8

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EP - 837

JO - Topics in Catalysis

JF - Topics in Catalysis

IS - 2

ER -

Veal heart ribonuclease P has an essential RNA component

Abstract

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