Variable spontaneous mutation and loss of heterozygosity among heterozygous genomes in yeast

Duong T. Nguyen, Baojun Wu, Hongan Long, Nan Zhang, Caitlyn Patterson, Stephen Simpson, Krystalynne Morris, W. Kelley Thomas, Michael Lynch, Weilong Hao

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Mutation and recombination are the primary sources of genetic variation. To better understand the evolution of genetic variation, it is crucial to comprehensively investigate the processes involving mutation accumulation and recombination. In this study, we performed mutation accumulation experiments on four heterozygous diploid yeast species in the Saccharomycodaceae family to determine spontaneous mutation rates, mutation spectra, and losses of heterozygosity (LOH). We observed substantial variation in mutation rates and mutation spectra. We also observed high LOH rates (1.65-11.07×10-6 events per heterozygous site per cell division). Biases in spontaneous mutation and LOH together with selection ultimately shape the variable genome-wide nucleotide landscape in yeast species.

Original languageEnglish (US)
Pages (from-to)3118-3130
Number of pages13
JournalMolecular biology and evolution
Issue number11
StatePublished - Nov 1 2020


  • Base substitutions
  • Diploid genome
  • GC content
  • LOH
  • Mutation rate
  • Mutational spectrum

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics


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