Vaccines prevent reinduction of rheumatoid arthritis symptoms in collagen-induced arthritis mouse model

Abhirami Thumsi, Srivatsan J. Swaminathan, Joslyn L. Mangal, Abhirami P. Suresh, Abhinav P. Acharya

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Metabolic reprogramming of immune cells modulates their function and reduces the severity of autoimmune diseases. However, the long-term effects of the metabolically reprogrammed cells, specifically in the case of immune flare-ups, need to be examined. Herein, a re-induction rheumatoid arthritis (RA) mouse model was developed by injecting T-cells from RA mice into drug-treated mice to recapitulate the effects of T-cell-mediated inflammation and mimic immune flare-ups. Immune metabolic modulator paKG(PFK15 + bc2) microparticles (MPs) were shown to reduce clinical symptoms of RA in collagen-induced arthritis (CIA) mice. Upon re-induction, a significant delay in the reappearance of clinical symptoms in the paKG(PFK15 + bc2) microparticle treatment group was observed as compared to equal or higher doses of the clinically utilized U.S. Food and Drug Administration (FDA)-approved drug, Methotrexate (MTX). Furthermore, paKG(PFK15 + bc2) microparticle-treated mice were able to lower activated dendritic cells (DCs) and inflammatory T helper cell 1 (TH1) and increased activated, proliferating regulatory T-cells (Tregs) more effectively than MTX. The paKG(PFK15 + bc2) microparticles also led to a significant reduction in paw inflammation in mice as compared to MTX treatment. This study can pave the way for the development of flare-up mouse models and antigen-specific drug treatments. Graphical Abstract: [Figure not available: see fulltext.]

Original languageEnglish (US)
Pages (from-to)1925-1935
Number of pages11
JournalDrug Delivery and Translational Research
Issue number7
StatePublished - Jul 2023


  • Collagen-induced arthritis
  • Immunoengineering
  • Immunometabolism
  • Reinduction
  • Rheumatoid arthritis
  • T cells

ASJC Scopus subject areas

  • Pharmaceutical Science


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