TY - JOUR
T1 - Understanding the polymorphic behaviour of a mutant of the -spectrin SH3 domain by means of two 1.1 Å resolution structures
AU - Cámara-Artigas, Ana
AU - Gavira, Jose Antonio
AU - Casares, Salvador
AU - Garcia-Ruiz, Juan Manuel
AU - Conejero-Lara, Francisco
AU - Allen, James
AU - Martinez, Jose C.
PY - 2011/3/1
Y1 - 2011/3/1
N2 - SH3 domains are small protein modules that mediate the assembly of specific protein complexes, typically via binding to proline-rich sequences in their respective binding partners. Most of the -spectrin SH3-domain (Spc-SH3) structures determined to date using X-ray diffraction have been solved from crystals belonging to the orthorhombic space group P212121 with a needle-like morphology. All of these ortho-rhombic crystals exhibited a rapid growth rate. In addition to this crystal form, the R21D mutant of Spc-SH3 crystallizes in a new crystal form in the presence of sodium formate at pH values higher than 6. This new crystal form grows at a slower rate and belongs to the hexagonal space group P6522, with unit-cell parameters a = b = 42.9, c = 127.5 Å. When both polymorphs of the R21D mutant of Spc-SH3 are simultaneously present into the same solution, it has been observed that the hexagonal crystals grow at the expense of the orthorhombic crystals. The availability of 1.1 Å resolution structures for both crystal forms allows the identification of key features that could account for the observed polymorphic behaviour.
AB - SH3 domains are small protein modules that mediate the assembly of specific protein complexes, typically via binding to proline-rich sequences in their respective binding partners. Most of the -spectrin SH3-domain (Spc-SH3) structures determined to date using X-ray diffraction have been solved from crystals belonging to the orthorhombic space group P212121 with a needle-like morphology. All of these ortho-rhombic crystals exhibited a rapid growth rate. In addition to this crystal form, the R21D mutant of Spc-SH3 crystallizes in a new crystal form in the presence of sodium formate at pH values higher than 6. This new crystal form grows at a slower rate and belongs to the hexagonal space group P6522, with unit-cell parameters a = b = 42.9, c = 127.5 Å. When both polymorphs of the R21D mutant of Spc-SH3 are simultaneously present into the same solution, it has been observed that the hexagonal crystals grow at the expense of the orthorhombic crystals. The availability of 1.1 Å resolution structures for both crystal forms allows the identification of key features that could account for the observed polymorphic behaviour.
KW - Ostwalds rule
KW - SH3 domains
KW - polymorphism
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U2 - 10.1107/S0907444911001715
DO - 10.1107/S0907444911001715
M3 - Article
C2 - 21358049
AN - SCOPUS:79952269718
SN - 0907-4449
VL - 67
SP - 189
EP - 196
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
IS - 3
ER -