TY - JOUR
T1 - Ultrahigh-cell-density culture of a marine green alga Chlorococcum littorale in a flat-plate photobioreactor
AU - Hu, Q.
AU - Kurano, N.
AU - Kawachi, M.
AU - Iwasaki, I.
AU - Miyachi, S.
N1 - Funding Information:
Acknowledgements We wish to thank Amos Richmond for a critical reading of the manuscript, Hiroshi Urata for help with the electron microscopy and Chiaki Komukai for technical assistance. This work was supported in part by a grant from the New Energy and Industial Technology Development Organization (NEDO). The experiments comply with the current laws of Japan in which the experiments were performed.
PY - 1998
Y1 - 1998
N2 - To test the feasibility of CO2 remediation by microalgal photosynthesis, a modified type of flat-plate photobioreactol [Hu et al. (1996) Biotechnol Bioeng 51:51-60] has been designed for cultivation of a high-CO2-tolerant unicellular green alga Chlorococcum littorale. The modified reactor has a narrow light path in which intensive turbulent flow is provided by streaming compressed air through perforated tubing into the culture suspension. The length of the reactor light path was optimized for the productivity of biomass. The interrelationship between cell density and productivity, as affected by incident light intensity, was quantitatively assessed, cellular ultrastructural and biochemical changes in response to ultrahigh cell density were investigated. The potential of biomass production under extremely high CO2 concentrations was also evaluated. By growing C. littorale cells in this reactor, a CO2 fixation rate of 16.7 g CO2 l-1 24 h-1 (or 200.4 g CO2 m-2 14 h-1) could readily be sustained at a light intensity of 2000 μmol m-2 s-1 at 25°C, and an ultrahigh cell density of well over 80 g l-1 could be maintained by daily replacing the culture medium.
AB - To test the feasibility of CO2 remediation by microalgal photosynthesis, a modified type of flat-plate photobioreactol [Hu et al. (1996) Biotechnol Bioeng 51:51-60] has been designed for cultivation of a high-CO2-tolerant unicellular green alga Chlorococcum littorale. The modified reactor has a narrow light path in which intensive turbulent flow is provided by streaming compressed air through perforated tubing into the culture suspension. The length of the reactor light path was optimized for the productivity of biomass. The interrelationship between cell density and productivity, as affected by incident light intensity, was quantitatively assessed, cellular ultrastructural and biochemical changes in response to ultrahigh cell density were investigated. The potential of biomass production under extremely high CO2 concentrations was also evaluated. By growing C. littorale cells in this reactor, a CO2 fixation rate of 16.7 g CO2 l-1 24 h-1 (or 200.4 g CO2 m-2 14 h-1) could readily be sustained at a light intensity of 2000 μmol m-2 s-1 at 25°C, and an ultrahigh cell density of well over 80 g l-1 could be maintained by daily replacing the culture medium.
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U2 - 10.1007/s002530051228
DO - 10.1007/s002530051228
M3 - Article
AN - SCOPUS:0031809302
SN - 0175-7598
VL - 49
SP - 655
EP - 662
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 6
ER -