TY - GEN
T1 - Two-phase mini-separator to separate alpha-fetoprotein from fibrinogen and immunoglobulin
AU - Yang, Yongmo
AU - Chae, Junseok
PY - 2009
Y1 - 2009
N2 - This paper reports a two-phase mini-column separator that depletes Immunoglobulin G (IgG) and separates α-fetoProtein (AFP) and fibrinogen. A mixture of IgG, fibrinogen, and AFP goes through the two-phase separator, where IgG is depleted by an affinity mini-column using Protein A/G agarose beads in the first phase. In the second phase, AFP is separated from fibrinogen by Size Exclusion Chromatography (SEC). The IgG depletion and SEC separation results are confirmed by evaluating fluorescent intensity of the eluents. Red (IgG), green (AFP), and blue (fibrinogen) intensities show that over 99.5% of IgG is depleted and, in 10 min, the second phase SEC minicolumn separates AFP (1 μg/mL) from fibrinogen-rich solution (10 μg/mL) with 2.78 resolution and 0.0056 mm plate height.
AB - This paper reports a two-phase mini-column separator that depletes Immunoglobulin G (IgG) and separates α-fetoProtein (AFP) and fibrinogen. A mixture of IgG, fibrinogen, and AFP goes through the two-phase separator, where IgG is depleted by an affinity mini-column using Protein A/G agarose beads in the first phase. In the second phase, AFP is separated from fibrinogen by Size Exclusion Chromatography (SEC). The IgG depletion and SEC separation results are confirmed by evaluating fluorescent intensity of the eluents. Red (IgG), green (AFP), and blue (fibrinogen) intensities show that over 99.5% of IgG is depleted and, in 10 min, the second phase SEC minicolumn separates AFP (1 μg/mL) from fibrinogen-rich solution (10 μg/mL) with 2.78 resolution and 0.0056 mm plate height.
KW - Plasma
KW - Sample preparation
KW - Separator
KW - Size exclusion chromatography
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U2 - 10.1109/SENSOR.2009.5285552
DO - 10.1109/SENSOR.2009.5285552
M3 - Conference contribution
AN - SCOPUS:71449097972
SN - 9781424441938
T3 - TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
SP - 104
EP - 107
BT - TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
T2 - TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems
Y2 - 21 June 2009 through 25 June 2009
ER -