Abstract
Bioelectrochemical performance of bacterial communities of cropland soil and lake sediment was evaluated in double chamber microbial fuel cells (MFCs) under the effect of trivalent iron [Fe(III)]. The MFCs were operated in a fed batch mode and their voltage output was measured for a period of 40 days. The highest outputs recorded under untreated conditions using cropland soil and lake sediment were 154 mV (current density= 0.06 mAcm-2, power density = 9.5 mWcm-2) and 137 mV (current density; 0.06 mAcm-2, power density; 7.5 mWcm-2), respectively. An about 87.7% (voltage; 289 mV, power density; 33.4 mWcm-2) and 45% (voltage: 160 mV, current density; 0.06 mAcm-2, power density; 10.24 mWcm-2) increase in voltage output was measured in the respective soil and sediment inoculated MFCs under iron treatment. The COD removal rate increased from 40% and 35-69% and 65% under treated conditions. Alpha rarefaction curves and Shannon index revealed soil derived biofilms contained the most diverse bacterial communities and diversity significantly reduced in sediment biofilm treated with Fe3+. Illumina MiSeq sequencing and scanning electron microscopy indicated the presence of a diverse microbial diversity where Proteobacteria, Bacteriodetes and Firmicutes were the most abundant phyla. Predominant genera included Pseudomonas, Sedimentibacter, Aminobacterium, Clostridium and Flavobacterium sp. MFCs with sediment supported the delta-proteobacteria; the class that includes Shewanella sp. and Geobacter sp., higher in the presence of Fe3+ than soil-MFCs. Based on 16SrRNA sequencing, isolated bacteria were identified as Staphylococcus sp., Bacillus sp., Streptomyces sp. and Gordonia sp., already reported for their electricity generation potential.
Original language | English (US) |
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Article number | 107790 |
Journal | Journal of Environmental Chemical Engineering |
Volume | 10 |
Issue number | 3 |
DOIs | |
State | Published - Jun 2022 |
Keywords
- Cropland soil
- Electrogenic bacterial communities
- Ferric iron [Fe(III)]
- Illumina Miseq sequencing
- Lake sediment
- Microbial fuel cell
ASJC Scopus subject areas
- Chemical Engineering (miscellaneous)
- Waste Management and Disposal
- Pollution
- Process Chemistry and Technology