Transforming growth factor α regulation of two zinc finger-containing immediate early response genes in intestine

R. N. DuBois, P. R. Bishop, R. Graves-Deal, R. J. Coffey

    Research output: Contribution to journalArticlepeer-review

    12 Scopus citations

    Abstract

    The epithelium lining the intestine undergoes rapid and continuous renewal. Growth factors play a role in intestinal epithelial growth regulation in vitro and in vivo. In this study, transforming growth factor α (TGFα) is shown to act as a mitogen and induce the expression of two zinc finger-containing immediate early genes [Zif268 (zinc finger protein 268) and Nup475 (nuclear protein 475)] in rat intestinal epithelial (RIE-1) cells in culture. These two gene products were initially isolated from serum-treated fibroblasts and represent growth-stimulated transcription factors. In TGFα- treated RIE-1 cells, nuclear run-on experiments demonstrate that TGFα induction of these two genes is regulated predominantly at the level of gene transcription. Utilizing in situ hybridization techniques, we show that systemic administration of TGFα induces expression of these two genes in the rat intestine. The predominant expression of zif268 is observed in the proliferative crypt compartment, whereas nup475 expression is concentrated in the postmitotic luminal compartment. These studies demonstrate that two immediate early genes, Nup475 and Zif268, are induced in intestinal epithelium in vitro and in vivo and thus may play a role in intestinal epithelial growth and/or differentiation.

    Original languageEnglish (US)
    Pages (from-to)523-529
    Number of pages7
    JournalCell Growth and Differentiation
    Volume6
    Issue number5
    StatePublished - 1995

    ASJC Scopus subject areas

    • Molecular Biology
    • Cell Biology

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