Time-resolved single molecule microscopy coupled with atomic force microscopy

M. König, F. Koberling, O. Schulz, Robert Ros, S. Fore, M. Sackrow, S. Trautmann, R. Erdmann

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations


Time-resolved confocal microscopy is well established to image spectral and spatial properties of samples in biology and material science. Atomic Force Microscopy (AFM) in addition enables to investigate properties which are not optically addressable or are hidden by the diffraction limited optical resolution. We present a straight forward combination of single molecule sensitive time-resolved confocal microscopy with different commercially available AFMs. Besides an extra of information about for example a cell surface, the AFM tip can also be used to manipulate the sample on a nanometer scale down to the single molecule level.

Original languageEnglish (US)
Title of host publicationThree-Dimensional and Multidimensional Microscopy
Subtitle of host publicationImage Acquisition and Processing XIX
StatePublished - Apr 16 2012
EventThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX - San Francisco, CA, United States
Duration: Jan 24 2012Jan 26 2012

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX
Country/TerritoryUnited States
CitySan Francisco, CA


  • AFM
  • Atomic Force Microscopy
  • Confocal microscopy
  • fluorescence
  • quenching
  • single molecule
  • sub diffraction
  • time-resolved

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging


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