Third generation impedimetric sensor employing direct electron transfer type glucose dehydrogenase

Yuka Ito, Junko Okuda-Shimazaki, Wakako Tsugawa, Noya Loew, Isao Shitanda, Chi En Lin, Jeffrey LaBelle, Koji Sode

Research output: Contribution to journalArticlepeer-review

38 Scopus citations


Faradaic electrochemical impedance spectroscopy (faradaic EIS) is an attractive measurement principle for biosensors. However, there have been no reports on sensors employing direct electron transfer (DET)-type redox enzymes based on faradaic EIS principle. In this study, we have attempted to construct the 3rd-generation faradaic enzyme EIS sensor, which used DET-type flavin adenine dinucleotide (FAD) dependent glucose dehydrogenase (GDH) complex, to elucidate its characteristic properties as well as to investigate its potential application as the future immunosensor platform. The gold disk electrodes (GDEs) with DET-type FADGDH prepared using self-assembled monolayer (SAM) showed the glucose concentration dependent impedance change, which was confirmed by the change in the charge transfer resistance (R ct ). The Δ(1/R ct ) values were also affected by DC bias potential and the length of SAM. Based on the Nyquist plot and Bode plot simulations, glucose sensing by imaginary impedance monitoring under fixed frequency (5 mHz) was carried out, revealing the higher sensitivity at low glucose concentration with wider linear range (0.02–0.2 mM). Considering this high sensitivity toward glucose, the 3rd-generation faradaic enzyme EIS sensor would provide alternative platform for future impedimetric immunosensing system, which does not use redox probe.

Original languageEnglish (US)
Pages (from-to)189-197
Number of pages9
JournalBiosensors and Bioelectronics
StatePublished - Mar 15 2019


  • Charge transfer resistance
  • Direct electron transfer
  • FAD dependent glucose dehydrogenase complex
  • Faradaic electrochemical impedance spectroscopy
  • Imaginary impedance monitoring
  • Impedimetric biosensor

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry


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