TY - JOUR
T1 - The irreversible inactivation of two copper-dependent monooxygenases by sulfite
T2 - peptidylglycine α-amidating enzyme and dopamine β-monooxygenase
AU - Merkler, David J.
AU - Kulathila, Raviraj
AU - Francisco, Wilson A.
AU - Ash, David E.
AU - Bell, Joseph
N1 - Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 1995/6/12
Y1 - 1995/6/12
N2 - Peptidylglycine α-amidating enzyme (α-AE) and dopamine β-monooxygenase (DβM), two copper-dependent monooxygenases that have catalytic and structural similarities, are irreversibly inactivated by sodium sulfite in a time- and concentration-dependent manner. Studies with α-AE show that the sulfite-mediated inactivation is dependent on the presence of redox active transition metals free in solution, with Cu(II) being the most effective in supporting the inactivation reaction. Sulfite inactivation of α-AE is specific for the monooxygenase reaction of this bifunctional enzyme and amidated peptides provide protection against the inactivation. Consequently, the sulfite-mediated inactivation of α-AE and DβM most likely results from the transition metal-catalyzed oxidation of sulfite to the sulfite radical, SO3-.
AB - Peptidylglycine α-amidating enzyme (α-AE) and dopamine β-monooxygenase (DβM), two copper-dependent monooxygenases that have catalytic and structural similarities, are irreversibly inactivated by sodium sulfite in a time- and concentration-dependent manner. Studies with α-AE show that the sulfite-mediated inactivation is dependent on the presence of redox active transition metals free in solution, with Cu(II) being the most effective in supporting the inactivation reaction. Sulfite inactivation of α-AE is specific for the monooxygenase reaction of this bifunctional enzyme and amidated peptides provide protection against the inactivation. Consequently, the sulfite-mediated inactivation of α-AE and DβM most likely results from the transition metal-catalyzed oxidation of sulfite to the sulfite radical, SO3-.
KW - Copper-dependent monooxygenase
KW - Dopamine hydroxylation
KW - Peptide α-amidation
KW - Sulfite-mediated inactivation
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U2 - 10.1016/0014-5793(95)00516-C
DO - 10.1016/0014-5793(95)00516-C
M3 - Article
C2 - 7540562
AN - SCOPUS:0029030649
SN - 0014-5793
VL - 366
SP - 165
EP - 169
JO - FEBS Letters
JF - FEBS Letters
IS - 2-3
ER -