The expression and genomic organization of randomly selected cloned Drosophila melanogaster genes

Elliott S. Goldstein; Walter S. Vincent; Kathleen A. Schultz

doi:10.1016/0167-4781(86)90036-9

The expression and genomic organization of randomly selected cloned Drosophila melanogaster genes

Elliott S. Goldstein, Walter S. Vincent, Kathleen A. Schultz

Life Sciences, School of (SOLS)

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

A lambda recombinant DNA library containing Drosophila melanogaster nuclear DNA inserts was screened with cDNA made from oocyte and gastrula poly(A)⁺ RNA. 124 clones were isolated which represented sequences complementary to a distribution of abundancies of their RNAs. The clone set was then used as probes to identify those whose RNA abundancies changed during embryonic development. The vast majority of clones showed little difference during development. Four different clones were identified whose poly(A)⁺ RNAs were quantitatively regulated; two were oocyte-specific, and two were embryonic-specific. 44 clones were chosen for in situ hybridization to salivary gland polytene chromosomes. The location and distribution of their sites are described. A class of clones, identified by in situ hybridization to the nucleolus, is further described. These clones contain a scrambled array of ribosomal intervening sequences.

Original language	English (US)
Pages (from-to)	209-219
Number of pages	11
Journal	BBA - Gene Structure and Expression
Volume	867
Issue number	4
DOIs	https://doi.org/10.1016/0167-4781(86)90036-9
State	Published - Aug 22 1986

Keywords

(D melanogaster)
Development
Embyrogenesis
In situ hybridization
mRNA population

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/0167-4781(86)90036-9

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title = "The expression and genomic organization of randomly selected cloned Drosophila melanogaster genes",

abstract = "A lambda recombinant DNA library containing Drosophila melanogaster nuclear DNA inserts was screened with cDNA made from oocyte and gastrula poly(A)+ RNA. 124 clones were isolated which represented sequences complementary to a distribution of abundancies of their RNAs. The clone set was then used as probes to identify those whose RNA abundancies changed during embryonic development. The vast majority of clones showed little difference during development. Four different clones were identified whose poly(A)+ RNAs were quantitatively regulated; two were oocyte-specific, and two were embryonic-specific. 44 clones were chosen for in situ hybridization to salivary gland polytene chromosomes. The location and distribution of their sites are described. A class of clones, identified by in situ hybridization to the nucleolus, is further described. These clones contain a scrambled array of ribosomal intervening sequences.",

keywords = "(D melanogaster), Development, Embyrogenesis, In situ hybridization, mRNA population",

author = "Goldstein, {Elliott S.} and Vincent, {Walter S.} and Schultz, {Kathleen A.}",

note = "Funding Information: We would like to thank Dr. Norman Davidson for the gift of the Drosophila Lambda library and Dr. David Glover for providing the clones pDm238, pc225 and pSR145. This project was begun in the laboratory of Dr. Eliot Myerowitz and we are grateful for his help. This research was supported in part by N.I.H. grant HD09157 and by Biomedical Research Support Grant S07 RR07112.",

year = "1986",

month = aug,

day = "22",

doi = "10.1016/0167-4781(86)90036-9",

language = "English (US)",

volume = "867",

pages = "209--219",

journal = "BBA - Gene Structure and Expression",

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TY - JOUR

T1 - The expression and genomic organization of randomly selected cloned Drosophila melanogaster genes

AU - Goldstein, Elliott S.

AU - Vincent, Walter S.

AU - Schultz, Kathleen A.

N1 - Funding Information: We would like to thank Dr. Norman Davidson for the gift of the Drosophila Lambda library and Dr. David Glover for providing the clones pDm238, pc225 and pSR145. This project was begun in the laboratory of Dr. Eliot Myerowitz and we are grateful for his help. This research was supported in part by N.I.H. grant HD09157 and by Biomedical Research Support Grant S07 RR07112.

PY - 1986/8/22

Y1 - 1986/8/22

N2 - A lambda recombinant DNA library containing Drosophila melanogaster nuclear DNA inserts was screened with cDNA made from oocyte and gastrula poly(A)+ RNA. 124 clones were isolated which represented sequences complementary to a distribution of abundancies of their RNAs. The clone set was then used as probes to identify those whose RNA abundancies changed during embryonic development. The vast majority of clones showed little difference during development. Four different clones were identified whose poly(A)+ RNAs were quantitatively regulated; two were oocyte-specific, and two were embryonic-specific. 44 clones were chosen for in situ hybridization to salivary gland polytene chromosomes. The location and distribution of their sites are described. A class of clones, identified by in situ hybridization to the nucleolus, is further described. These clones contain a scrambled array of ribosomal intervening sequences.

AB - A lambda recombinant DNA library containing Drosophila melanogaster nuclear DNA inserts was screened with cDNA made from oocyte and gastrula poly(A)+ RNA. 124 clones were isolated which represented sequences complementary to a distribution of abundancies of their RNAs. The clone set was then used as probes to identify those whose RNA abundancies changed during embryonic development. The vast majority of clones showed little difference during development. Four different clones were identified whose poly(A)+ RNAs were quantitatively regulated; two were oocyte-specific, and two were embryonic-specific. 44 clones were chosen for in situ hybridization to salivary gland polytene chromosomes. The location and distribution of their sites are described. A class of clones, identified by in situ hybridization to the nucleolus, is further described. These clones contain a scrambled array of ribosomal intervening sequences.

KW - (D melanogaster)

KW - Development

KW - Embyrogenesis

KW - In situ hybridization

KW - mRNA population

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DO - 10.1016/0167-4781(86)90036-9

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AN - SCOPUS:0022545461

SN - 0167-4781

VL - 867

SP - 209

EP - 219

JO - BBA - Gene Structure and Expression

JF - BBA - Gene Structure and Expression

IS - 4

ER -

The expression and genomic organization of randomly selected cloned Drosophila melanogaster genes

Abstract

Keywords

ASJC Scopus subject areas

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