TY - JOUR
T1 - The Complete Protist Symbiont Communities of Coptotermes formosanus and Coptotermes gestroi
T2 - Morphological and Molecular Characterization of Five New Species
AU - Jasso-Selles, Daniel E.
AU - De Martini, Francesca
AU - Velenovsky, Joseph F.
AU - Mee, Evan D.
AU - Montoya, Samantha J.
AU - Hileman, Jonathon T.
AU - Garcia, Mikaela D.
AU - Su, Nan Yao
AU - Chouvenc, Thomas
AU - Gile, Gillian H.
N1 - Funding Information:
The authors would like to thank Trevor Merrell for technical assistance with protist molecular work and Zachary Kaplan, Alvin Puzio, and Reynaldo Moscat for termite colony maintenance. This work was supported mainly by NSF grants DEB‐1754083 and DEB‐1754337, and in part by USDA National Institute of Food and Agriculture, Hatch project numbers FLA‐FTL 005865 and FLA‐FLT 005660, a University of Florida Early Career Seed Grant No. REA1801100, and the School of Life Sciences and College of Liberal Arts and Sciences at Arizona State University (startup grant to GHG).
Publisher Copyright:
© 2020 International Society of Protistologists
PY - 2020/11/1
Y1 - 2020/11/1
N2 - Coptotermes formosanus Shiraki and Coptotermes gestroi (Wasmann) (Blattoidea: Rhinotermitidae) are invasive subterranean termite pest species with a major global economic impact. However, the descriptions of the mutualistic protist communities harbored in their respective hindguts remain fragmentary. The C. formosanus hindgut has long been considered to harbor three protist species, Pseudotrichonympha grassii (Trichonymphida), Holomastigotoides hartmanni, and Cononympha (Spirotrichonympha) leidyi (Spirotrichonymphida), but molecular data have suggested that the diversity may be higher. Meanwhile, the C. gestroi community remains undescribed except for Pseudotrichonympha leei. To complete the characterization of these communities, hindguts of workers from both termite species were investigated using single-cell PCR, microscopy, cell counts, and 18S rRNA amplicon sequencing. The two hosts were found to harbor intriguingly parallel protist communities, each consisting of one Pseudotrichonympha species, two Holomastigotoides species, and two Cononympha species. All protist species were unique to their respective hosts, which last shared a common ancestor ~18 MYA. The relative abundances of protist species in each hindgut differed remarkably between cell count data and 18S rRNA profiles, calling for caution in interpreting species abundances from amplicon data. This study will enable future research in C. formosanus and C. gestroi hybrids, which provide a unique opportunity to study protist community inheritance, compatibility, and potential contribution to hybrid vigor.
AB - Coptotermes formosanus Shiraki and Coptotermes gestroi (Wasmann) (Blattoidea: Rhinotermitidae) are invasive subterranean termite pest species with a major global economic impact. However, the descriptions of the mutualistic protist communities harbored in their respective hindguts remain fragmentary. The C. formosanus hindgut has long been considered to harbor three protist species, Pseudotrichonympha grassii (Trichonymphida), Holomastigotoides hartmanni, and Cononympha (Spirotrichonympha) leidyi (Spirotrichonymphida), but molecular data have suggested that the diversity may be higher. Meanwhile, the C. gestroi community remains undescribed except for Pseudotrichonympha leei. To complete the characterization of these communities, hindguts of workers from both termite species were investigated using single-cell PCR, microscopy, cell counts, and 18S rRNA amplicon sequencing. The two hosts were found to harbor intriguingly parallel protist communities, each consisting of one Pseudotrichonympha species, two Holomastigotoides species, and two Cononympha species. All protist species were unique to their respective hosts, which last shared a common ancestor ~18 MYA. The relative abundances of protist species in each hindgut differed remarkably between cell count data and 18S rRNA profiles, calling for caution in interpreting species abundances from amplicon data. This study will enable future research in C. formosanus and C. gestroi hybrids, which provide a unique opportunity to study protist community inheritance, compatibility, and potential contribution to hybrid vigor.
KW - Cononympha
KW - Holomastigotoides
KW - Metamonada
KW - Parabasalia
KW - Pseudotrichonympha
KW - Rhinotermitidae
KW - Spirotrichonympha
KW - hybrid
KW - microbiome
KW - termite
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U2 - 10.1111/jeu.12815
DO - 10.1111/jeu.12815
M3 - Article
C2 - 32603489
AN - SCOPUS:85088817320
SN - 1066-5234
VL - 67
SP - 626
EP - 641
JO - Journal of Eukaryotic Microbiology
JF - Journal of Eukaryotic Microbiology
IS - 6
ER -