Tension-induced neurite growth in microfluidic channels

Thanh D. Nguyen, Ian B. Hogue, Kellye Cung, Prashant K. Purohit, Michael C. McAlpine

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


The generation of an effective method for stimulating neuronal growth in specific directions, along well-defined geometries, and in numerous cells could impact areas ranging from fundamental studies of neuronal evolution and morphogenesis, to applications in biomedical diagnostics and nerve regeneration. Applied mechanical stress can regulate neurite growth. Indeed, previous studies have shown that neuronal cells can develop and extend neurites with rapid growth rates under applied "towing" tensions imparted by micropipettes. Yet, such methods are complex and exhibit low throughputs, as the tension is applied serially to individual cells. Here we present a novel approach to inducing neurite growth in multiple cells in parallel, by using a miniaturized platform with numerous microchannels. Upon connection of a vacuum to these microchannels, tension can be applied on multiple cells simultaneously to induce the growth of neurites. A theoretical model was also developed to understand the effect of tension on the dynamics of neurite development.

Original languageEnglish (US)
Pages (from-to)3735-3740
Number of pages6
JournalLab on a Chip
Issue number18
StatePublished - Sep 21 2013
Externally publishedYes

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • General Chemistry
  • Biomedical Engineering


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