Systemic and in vitro infection process of Bombyx mori nucleopolyhedrovirus

Md Masmudur Rahman, Karumathil P. Gopinathan

Research output: Contribution to journalArticlepeer-review

103 Scopus citations


To analyse the systemic progression of infection by Bombyx mori nucleopolyhedrovirus (BmNPV) through oral ingestion by the silkworm larvae, a recombinant virus (vBmp10GFP) expressing the green fluorescent protein (GFP) under the control of the very late, viral p10 promoter (which still forms the polyhedral occlusion bodies) was constructed. Infection of B. mori derived BmN cells with the recombinant virus resulted in the expression of GFP from 12h post infection (hpi), with maximal accumulation of the expressed protein by 60hpi. B. mori larvae that ingested the polyhedra containing vBmp10GFP showed localized expression of GFP in the midgut epithelial cells within 24hpi, indicating virus replication. The primary spread of the virus infection occurred through the tracheae. Viral multiplication was subsequently detected in nearly all the larval tissues including the neurons and regions of silk-glands that were in contact with the tracheae. Infection in fat bodies was widespread by 48hpi, by which time the haemocytes also showed infection. In vitro infection of isolated organs/tissues from B. mori with the budded virions (BV) of vBmp10GFP also showed viral multiplication in the cells that were associated with the tracheae, confirming the role of tracheae in spreading the infection.

Original languageEnglish (US)
Pages (from-to)109-118
Number of pages10
JournalVirus research
Issue number2
StatePublished - May 2004
Externally publishedYes


  • Baculovirus
  • BmNPV
  • Bombyx mori
  • GFP
  • Infection process
  • Silkworm
  • p10 promoter

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases


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