TY - JOUR
T1 - Synthetic long peptide booster immunization in rhesus macaques primed with replication-competent NYVAC-C-KC induces a balanced CD4/CD8 T-cell and antibody response against the conserved regions of HIV-1
AU - Mooij, Petra
AU - Koopman, Gerrit
AU - Drijfhout, Jan Wouter
AU - Nieuwenhuis, Ivonne G.
AU - Beenhakker, Niels
AU - Koestler, Josef
AU - Bogers, Willy M J M
AU - Wagner, Ralf
AU - Esteban, Mariano
AU - Pantaleo, Giuseppe
AU - Heeney, Jonathan L.
AU - Jacobs, Bertram
AU - Melief, Cornelis J M
N1 - Publisher Copyright:
© 2015 The Author.
PY - 2015
Y1 - 2015
N2 - The Thai trial (RV144) indicates that a prime–boost vaccine combination that induces both T-cell and antibody responses may be desirable for an effective HIV vaccine. We have previously shown that immunization with synthetic long peptides (SLP), covering the conserved parts of SIV, induced strong CD4 T-cell and antibody responses, but only modest CD8 T-cell responses. To generate a more balanced CD4/CD8 T-cell and antibody response, this study evaluated a poxvector prime/SLP boost strategy in rhesus macaques. Priming with a replication-competent NYVAC, encoding HIV-1 clade C gag, pol and nef, induced modest IFNc T-cell immune responses, predominantly directed against HIV-1 Gag. Booster immunization with SLP, covering the conserved parts of HIV-1 Gag, Pol and Env, resulted in a more than 10-fold increase in IFNc ELISpot responses in four of six animals, which were predominantly HIV-1 Pol-specific. The animals showed a balanced polyfunctional CD4 and CD8 T-cell response and high Ab titres.
AB - The Thai trial (RV144) indicates that a prime–boost vaccine combination that induces both T-cell and antibody responses may be desirable for an effective HIV vaccine. We have previously shown that immunization with synthetic long peptides (SLP), covering the conserved parts of SIV, induced strong CD4 T-cell and antibody responses, but only modest CD8 T-cell responses. To generate a more balanced CD4/CD8 T-cell and antibody response, this study evaluated a poxvector prime/SLP boost strategy in rhesus macaques. Priming with a replication-competent NYVAC, encoding HIV-1 clade C gag, pol and nef, induced modest IFNc T-cell immune responses, predominantly directed against HIV-1 Gag. Booster immunization with SLP, covering the conserved parts of HIV-1 Gag, Pol and Env, resulted in a more than 10-fold increase in IFNc ELISpot responses in four of six animals, which were predominantly HIV-1 Pol-specific. The animals showed a balanced polyfunctional CD4 and CD8 T-cell response and high Ab titres.
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U2 - 10.1099/vir.0.000074
DO - 10.1099/vir.0.000074
M3 - Article
C2 - 25667320
AN - SCOPUS:84937597034
SN - 0022-1317
VL - 96
SP - 1478
EP - 1483
JO - Journal of General Virology
JF - Journal of General Virology
ER -