Synthesis of pdCpAs and transfer RNAs activated with derivatives of aspartic acid and cysteine

Shengxi Chen; Sidney M. Hecht

doi:10.1016/j.bmc.2008.08.036

Synthesis of pdCpAs and transfer RNAs activated with derivatives of aspartic acid and cysteine

Shengxi Chen, Sidney M. Hecht

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Described herein is the preparation of new aminoacylated derivatives of the dinucleotide pdCpA, and of transfer RNAs. The focus of the present work is the synthesis of amino acid analogs related to aspartic acid and cysteine species that have important functional roles in many proteins. The activated aminoacyl-tRNAs prepared can be utilized for the elaboration of proteins containing modified aspartic acid and cysteine derivatives at predetermined sites. Of particular interest is definition of functional group protection strategies that can be used for the preparation of the aminoacylated pdCpAs and tRNAs.

Original language	English (US)
Pages (from-to)	9023-9031
Number of pages	9
Journal	Bioorganic and Medicinal Chemistry
Volume	16
Issue number	19
DOIs	https://doi.org/10.1016/j.bmc.2008.08.036
State	Published - Oct 1 2008
Externally published	Yes

Keywords

Aminoacylation
Enzymatic ligation
Protein synthesis
Sulfur-containing amino acids

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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10.1016/j.bmc.2008.08.036

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title = "Synthesis of pdCpAs and transfer RNAs activated with derivatives of aspartic acid and cysteine",

abstract = "Described herein is the preparation of new aminoacylated derivatives of the dinucleotide pdCpA, and of transfer RNAs. The focus of the present work is the synthesis of amino acid analogs related to aspartic acid and cysteine species that have important functional roles in many proteins. The activated aminoacyl-tRNAs prepared can be utilized for the elaboration of proteins containing modified aspartic acid and cysteine derivatives at predetermined sites. Of particular interest is definition of functional group protection strategies that can be used for the preparation of the aminoacylated pdCpAs and tRNAs.",

keywords = "Aminoacylation, Enzymatic ligation, Protein synthesis, Sulfur-containing amino acids",

author = "Shengxi Chen and Hecht, {Sidney M.}",

note = "Funding Information: This work was supported by NIH Research Grant CA77359, awarded by the National Cancer Institute.",

year = "2008",

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doi = "10.1016/j.bmc.2008.08.036",

language = "English (US)",

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TY - JOUR

T1 - Synthesis of pdCpAs and transfer RNAs activated with derivatives of aspartic acid and cysteine

AU - Chen, Shengxi

AU - Hecht, Sidney M.

N1 - Funding Information: This work was supported by NIH Research Grant CA77359, awarded by the National Cancer Institute.

PY - 2008/10/1

Y1 - 2008/10/1

N2 - Described herein is the preparation of new aminoacylated derivatives of the dinucleotide pdCpA, and of transfer RNAs. The focus of the present work is the synthesis of amino acid analogs related to aspartic acid and cysteine species that have important functional roles in many proteins. The activated aminoacyl-tRNAs prepared can be utilized for the elaboration of proteins containing modified aspartic acid and cysteine derivatives at predetermined sites. Of particular interest is definition of functional group protection strategies that can be used for the preparation of the aminoacylated pdCpAs and tRNAs.

AB - Described herein is the preparation of new aminoacylated derivatives of the dinucleotide pdCpA, and of transfer RNAs. The focus of the present work is the synthesis of amino acid analogs related to aspartic acid and cysteine species that have important functional roles in many proteins. The activated aminoacyl-tRNAs prepared can be utilized for the elaboration of proteins containing modified aspartic acid and cysteine derivatives at predetermined sites. Of particular interest is definition of functional group protection strategies that can be used for the preparation of the aminoacylated pdCpAs and tRNAs.

KW - Aminoacylation

KW - Enzymatic ligation

KW - Protein synthesis

KW - Sulfur-containing amino acids

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DO - 10.1016/j.bmc.2008.08.036

M3 - Article

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AN - SCOPUS:52949113008

SN - 0968-0896

VL - 16

SP - 9023

EP - 9031

JO - Bioorganic and Medicinal Chemistry

JF - Bioorganic and Medicinal Chemistry

IS - 19

ER -

Synthesis of pdCpAs and transfer RNAs activated with derivatives of aspartic acid and cysteine

Abstract

Keywords

ASJC Scopus subject areas

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