19F-MRS/1H-MRI dual-1 of β-galactosidase activity

Jian Xin Yu, Vikram Kodibagkar, Li Liu, Zhongwei Zhang, Jennifer Magnusson, Yuting Liu

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


Because of the importance of lacZ gene in various applications ranging from molecular biology to clinical trials, the development of non-invasive bimodal techniques for improving precision and accuracy to assay gene expression has attracted much attention. In this paper, we propose a dual-function probe for synergistic combination of 19F-MRS/1H-MRI to simultaneously detect β-gal activity. Based on this strategy, we have designed, synthesized and characterized a series of 19F-MRS/ 1H-MRI reporters, and demonstrated the feasibility of 1-O-(β-d-galactopyranosyl)-3-fluorocatechol MGD-3-FCAT for assessing β-gal activity in solution and in vitro with lacZ transfected tumor cells as well, by the characterization of β-gal responsive 19F- chemical shift changes ΔδF, hydrolytic kinetics, and T1, T2 relaxation mapping.

Original languageEnglish (US)
Pages (from-to)2132-2142
Number of pages11
JournalChemical Science
Issue number5
StatePublished - May 2013

ASJC Scopus subject areas

  • General Chemistry


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