TY - JOUR
T1 - Structures of proteins and cofactors
T2 - X-ray crystallography
AU - Allen, James
AU - Seng, C.
AU - Larson, C.
N1 - Funding Information:
Fig. 8 The three-dimensional structure of FMO from Chlorobium tepidum. Shown are the backbones of the three protein subunits (yellow, green, and blue) and the cofactors (black) of the trimer. The view is approximately down the threefold symmetry axis of the biologically active trimer. The coordinates are 1M50 (Camara-Artigas et al. 2003) Acknowledgment The work from our laboratory described in this review is supported from a grant from the National Science Foundation, MCB 0640002.
PY - 2009/11
Y1 - 2009/11
N2 - Protein crystallography is the predominately used technique for the determination of the three-dimensional structures of proteins and other macromolecules. In this article, the methodology utilized for the measurement and analysis of the diffraction data from crystals is briefly reviewed. As examples of both the usefulness and difficulties of this technique, the determination of the structures of several photosynthetic pigment-protein complexes is described, namely, the reaction center from purple bacteria, photosystem I and photosystem II from cyanobacteria, the light-harvesting complex II from purple bacteria, and the FMO protein from green bacteria.
AB - Protein crystallography is the predominately used technique for the determination of the three-dimensional structures of proteins and other macromolecules. In this article, the methodology utilized for the measurement and analysis of the diffraction data from crystals is briefly reviewed. As examples of both the usefulness and difficulties of this technique, the determination of the structures of several photosynthetic pigment-protein complexes is described, namely, the reaction center from purple bacteria, photosystem I and photosystem II from cyanobacteria, the light-harvesting complex II from purple bacteria, and the FMO protein from green bacteria.
KW - Light-harvesting complexes
KW - Photosystem I
KW - Photosystem II
KW - Protein crystallography
KW - Reaction centers
KW - X-ray diffraction
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U2 - 10.1007/s11120-009-9416-4
DO - 10.1007/s11120-009-9416-4
M3 - Review article
C2 - 19322673
AN - SCOPUS:76149090474
SN - 0166-8595
VL - 102
SP - 231
EP - 240
JO - Photosynthesis research
JF - Photosynthesis research
IS - 2
ER -