Structure determination of a DNA crystal by MicroED

Alison Haymaker; Andrey A. Bardin; Tamir Gonen; Michael W. Martynowycz; Brent L. Nannenga

doi:10.1016/j.str.2023.07.005

Structure determination of a DNA crystal by MicroED

Alison Haymaker, Andrey A. Bardin, Tamir Gonen, Michael W. Martynowycz, Brent L. Nannenga

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Microcrystal electron diffraction (MicroED) is a powerful tool for determining high-resolution structures of microcrystals from a diverse array of biomolecular, chemical, and material samples. In this study, we apply MicroED to DNA crystals, which have not been previously analyzed using this technique. We utilized the d(CGCGCG)₂ DNA duplex as a model sample and employed cryo-FIB milling to create thin lamella for diffraction data collection. The MicroED data collection and subsequent processing resulted in a 1.10 Å resolution structure of the d(CGCGCG)₂ DNA, demonstrating the successful application of cryo-FIB milling and MicroED to the investigation of nucleic acid crystals.

Original language	English (US)
Pages (from-to)	1499-1503.e2
Journal	Structure
Volume	31
Issue number	12
DOIs	https://doi.org/10.1016/j.str.2023.07.005
State	Published - Dec 7 2023

Keywords

DNA
cryo-electron microscopy
cryo-focused ion beam milling
microcrystal electron diffraction, MicroED
nucleic acids

ASJC Scopus subject areas

Structural Biology
Molecular Biology

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10.1016/j.str.2023.07.005

Cite this

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title = "Structure determination of a DNA crystal by MicroED",

abstract = "Microcrystal electron diffraction (MicroED) is a powerful tool for determining high-resolution structures of microcrystals from a diverse array of biomolecular, chemical, and material samples. In this study, we apply MicroED to DNA crystals, which have not been previously analyzed using this technique. We utilized the d(CGCGCG)2 DNA duplex as a model sample and employed cryo-FIB milling to create thin lamella for diffraction data collection. The MicroED data collection and subsequent processing resulted in a 1.10 {\AA} resolution structure of the d(CGCGCG)2 DNA, demonstrating the successful application of cryo-FIB milling and MicroED to the investigation of nucleic acid crystals.",

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T1 - Structure determination of a DNA crystal by MicroED

AU - Haymaker, Alison

AU - Bardin, Andrey A.

AU - Gonen, Tamir

AU - Martynowycz, Michael W.

AU - Nannenga, Brent L.

PY - 2023/12/7

Y1 - 2023/12/7

N2 - Microcrystal electron diffraction (MicroED) is a powerful tool for determining high-resolution structures of microcrystals from a diverse array of biomolecular, chemical, and material samples. In this study, we apply MicroED to DNA crystals, which have not been previously analyzed using this technique. We utilized the d(CGCGCG)2 DNA duplex as a model sample and employed cryo-FIB milling to create thin lamella for diffraction data collection. The MicroED data collection and subsequent processing resulted in a 1.10 Å resolution structure of the d(CGCGCG)2 DNA, demonstrating the successful application of cryo-FIB milling and MicroED to the investigation of nucleic acid crystals.

AB - Microcrystal electron diffraction (MicroED) is a powerful tool for determining high-resolution structures of microcrystals from a diverse array of biomolecular, chemical, and material samples. In this study, we apply MicroED to DNA crystals, which have not been previously analyzed using this technique. We utilized the d(CGCGCG)2 DNA duplex as a model sample and employed cryo-FIB milling to create thin lamella for diffraction data collection. The MicroED data collection and subsequent processing resulted in a 1.10 Å resolution structure of the d(CGCGCG)2 DNA, demonstrating the successful application of cryo-FIB milling and MicroED to the investigation of nucleic acid crystals.

KW - DNA

KW - cryo-electron microscopy

KW - cryo-focused ion beam milling

KW - microcrystal electron diffraction, MicroED

KW - nucleic acids

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Structure determination of a DNA crystal by MicroED

Abstract

Keywords

ASJC Scopus subject areas

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