TY - JOUR
T1 - Structure determination based on continuous diffraction from macromolecular crystals
AU - Chapman, Henry N.
AU - Fromme, Petra
N1 - Funding Information:
This work was funded by the European Research Council under the European Union's Seventh Framework Programme ERC Synergy Grant 609920 ‘Frontiers in Attosecond X-ray Science: Imaging and Spectroscopy (AXSIS)’. HNC additionally acknowledges project funds to DESY CFEL by the Helmholtz Association. PF acknowledges funding by the BioXFEL Science Technology Center (award 1231306); and the US National Institutes of Health ( NIH ), National Institute of General Medical Sciences grant R01 GM095583 .
Publisher Copyright:
© 2017
PY - 2017/8
Y1 - 2017/8
N2 - Bright and coherent X-ray sources, such free-electron lasers, have spurred large activities in developing new methods to obtain the structures of biological macromolecules. In particular, single-molecule diffraction is highly desired, as it would abolish the need for crystallization. It provides considerably more diffraction intensity information than needed to solve a structure, unlike crystal diffraction, which is usually insufficient for direct phasing. To overcome the challenge of weak scattering signals of single molecules, the direct phasing approaches in coherent diffractive imaging have been combined with crystals in several imaginative ways. One of these, using crystals with translational disorder, has been used to phase continuous femtosecond X-ray diffraction data from photosystem II complexes, offering a paradigm shift in crystallography.
AB - Bright and coherent X-ray sources, such free-electron lasers, have spurred large activities in developing new methods to obtain the structures of biological macromolecules. In particular, single-molecule diffraction is highly desired, as it would abolish the need for crystallization. It provides considerably more diffraction intensity information than needed to solve a structure, unlike crystal diffraction, which is usually insufficient for direct phasing. To overcome the challenge of weak scattering signals of single molecules, the direct phasing approaches in coherent diffractive imaging have been combined with crystals in several imaginative ways. One of these, using crystals with translational disorder, has been used to phase continuous femtosecond X-ray diffraction data from photosystem II complexes, offering a paradigm shift in crystallography.
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U2 - 10.1016/j.sbi.2017.07.008
DO - 10.1016/j.sbi.2017.07.008
M3 - Review article
C2 - 28917122
AN - SCOPUS:85029365859
SN - 0959-440X
VL - 45
SP - 170
EP - 177
JO - Current Opinion in Structural Biology
JF - Current Opinion in Structural Biology
ER -