@article{1c0108fe0356488e811ca57d9785ef0f,
title = "Structural and Functional Consequences of the Weak Binding of Chlorin e6 to Bovine Rhodopsin",
abstract = " The chlorophyll-derivative chlorin e6 (Ce6) identified in the retinas of deep-sea ocean fish is proposed to play a functional role in red bioluminescence detection. Fluorescence and 1 H NMR spectroscopy studies with the bovine dim-light photoreceptor, rhodopsin, indicate that Ce6 weakly binds to it with μm affinity. Absorbance spectra prove that red light sensitivity enhancement is not brought about by a shift in the absorbance maximum of rhodopsin. 19 F NMR experiments with samples where 19 F labels are either placed at the cytoplasmic binding site or incorporated as fluorinated retinal indicate that the cytoplasmic domain is highly perturbed by binding, while little to no changes are detected near the retinal. Binding of Ce6 also inhibits G-protein activation. Chemical shift changes in 1 H- 15 N NMR spectroscopy of 15 N-Trp labeled bovine rhodopsin reveal that Ce6 binding perturbs the entire structure. These results provide experimental evidence that Ce6 is an allosteric modulator of rhodopsin.",
author = "James Mitchell and Naveena Yanamala and Tan, {Yi Lei} and Gardner, {Eric E.} and Tirupula, {Kalyan C.} and Fernanda Balem and Mordechai Sheves and Daniel Nietlispach and Judith Klein-Seetharaman",
note = "Funding Information: Acknowledgements—This work was in part supported by an International Incoming Fellowship of the Seventh Framework Programme (FP7) Marie Curie Actions (to JKS), a fellowship by the DAAD-Helmholtz Society to FB, the Sofya Kovalevskaya Prize of the Humboldt Foundation, Germany/Zukunftsinvestitionsprogramm der Bundesregierung Deutschland, National Science Foundation grants EIA0225636 and CAREER CC044917 to JKS. We would like to thank Dr. Inja Byeon (Structural Biology, University of Pittsburgh) for help with the set-up of selective excitation NMR experiments. Funding Information: This work was in part supported by an International Incoming Fellowship of the Seventh Framework Programme (FP7) Marie?Curie Actions (to JKS), a fellowship by the DAAD-Helmholtz Society to FB, the Sofya Kovalevskaya Prize of the Humboldt Foundation, Germany/Zukunftsinvestitionsprogramm der Bundesregierung Deutschland, National Science Foundation grants EIA0225636 and CAREER CC044917 to JKS. We would like to thank Dr. Inja Byeon (Structural Biology, University of Pittsburgh) for help with the set-up of selective excitation NMR experiments. Publisher Copyright: {\textcopyright} 2018 American Society for Photobiology",
year = "2019",
month = may,
day = "1",
doi = "10.1111/php.13074",
language = "English (US)",
volume = "95",
pages = "787--802",
journal = "Photochemistry and photobiology",
issn = "0031-8655",
publisher = "Wiley-Blackwell",
number = "3",
}