@article{75ca98988d714406b2f03fae50633791,
title = "Spectroradiometric detection of competitor diatoms and the grazer Poteriochromonas in algal cultures",
abstract = "To address challenges in early detection of pond pests, we have extended a spectroradiometric monitoring method, initially demonstrated for measurement of pigment optical activity and biomass, to the detection of algal competitors and grazers. The method relies upon measurement and interpretation of pond reflectance spectra spanning from the visible into the near-infrared. Reflectance spectra are acquired every 5 min with a multi-channel, fiber-coupled spectroradiometer, providing monitoring of algal pond conditions with high temporal frequency. The spectra are interpreted via numerical inversion of a reflectance model, in which the above-water reflectance is expressed in terms of the absorption and backscatter coefficients of the cultured species, with additional terms accounting for the pigment fluorescence features and for the water-surface reflection of sunlight and skylight. With this method we demonstrate detection of diatoms and the predator Poteriochromonas in outdoor cultures of Nannochloropsis oceanica and Chlorella vulgaris, respectively. The relative strength of these signatures is compared to microscopy and sequencing analysis. Spectroradiometric detection of diatoms is then further assessed on beaker-contained mixtures of Microchloropsis salina with Phaeodactylum tricornutum, Thalassiosira weissflogii, and Thalassiosira pseudonana, respectively, providing an initial evaluation of the sensitivity and specificity of detecting pond competitors.",
keywords = "Algal crop protection, Algal cultivation, Reflectance spectroscopy",
author = "Reichardt, {Thomas A.} and Danae Maes and Jensen, {Travis J.} and Dempster, {Thomas A.} and McGowen, {John A.} and Kunal Poorey and Curtis, {Deanna J.} and Lane, {Todd W.} and Timlin, {Jerilyn A.}",
note = "Funding Information: The authors wish to thank Pierre Wensel for maintenance of the spectroradiometric equipment, Theresa Rosov for performing the microscopy and contamination counts and overseeing the cultivation experiment, Aaron Collins for his assistance with the initial set-up of the monitoring equipment at AzCATI, and Tyler Hipple for assistance with the spectroradiometric analysis of the pond data. This work was funded by the U.S. Department of Energy, Bioenergy Technologies Office (BETO), through project DE-EE0005996, the Algae Testbed Public Private Partnership (data collection, software tool development) and project NL0032356 (DISCOVR, data analysis and interpretation). Sandia National Laboratories is a multimission laboratory managed and operated by National Technology & Engineering Solutions of Sandia, LLC, a wholly owned subsidiary of Honeywell International Inc. for the U.S. Department of Energy's National Nuclear Security Administration under contract DE-NA0003525. This paper describes objective technical results and analysis. Any subjective views or opinions that might be expressed in the paper do not necessarily represent the views of the U.S. Department of Energy or the United States Government. No conflicts, informed consent, or human or animal rights are applicable to this study. Funding Information: The authors wish to thank Pierre Wensel for maintenance of the spectroradiometric equipment, Theresa Rosov for performing the microscopy and contamination counts and overseeing the cultivation experiment, Aaron Collins for his assistance with the initial set-up of the monitoring equipment at AzCATI, and Tyler Hipple for assistance with the spectroradiometric analysis of the pond data. This work was funded by the U.S. Department of Energy, Bioenergy Technologies Office (BETO), through project DE-EE0005996 , the Algae Testbed Public Private Partnership (data collection, software tool development) and project NL0032356 (DISCOVR, data analysis and interpretation). Sandia National Laboratories is a multimission laboratory managed and operated by National Technology & Engineering Solutions of Sandia, LLC, a wholly owned subsidiary of Honeywell International Inc., for the U.S. Department of Energy's National Nuclear Security Administration under contract DE-NA0003525. This paper describes objective technical results and analysis. Any subjective views or opinions that might be expressed in the paper do not necessarily represent the views of the U.S. Department of Energy or the United States Government. Publisher Copyright: {\textcopyright} 2020 Elsevier B.V.",
year = "2020",
month = oct,
doi = "10.1016/j.algal.2020.102020",
language = "English (US)",
volume = "51",
journal = "Algal Research",
issn = "2211-9264",
publisher = "Elsevier BV",
}