Single-molecule fluorescence studies of IDPs and IDRs

Wenwei Zheng, Hoi Sung Chung

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations


Within the arsenal of experimental methods in studying intrinsically disordered proteins (IDPs), single-molecule fluorescence methods provide unique long-range distance and dynamics information. In this chapter, we discuss recent progress on using single-molecule fluorescence methods for probing both intra- and intermolecular interactions of IDPs. We focus on several aspects, including models for analyzing experimental data, physical principles that can be obtained, and combinations with other experimental methods for a comprehensive view of the structural properties of IDPs.

Original languageEnglish (US)
Title of host publicationIntrinsically Disordered Proteins
Subtitle of host publicationDynamics, Binding, and Function
Number of pages44
ISBN (Electronic)9780128163481
ISBN (Print)9780128167328
StatePublished - Jan 1 2019
Externally publishedYes


  • Fluorescence correlation spectroscopy
  • Molecule dynamics simulation
  • Polymer model
  • Protein dynamics
  • Single-molecule Förster resonance energy transfer

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology


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