Abstract
Within the arsenal of experimental methods in studying intrinsically disordered proteins (IDPs), single-molecule fluorescence methods provide unique long-range distance and dynamics information. In this chapter, we discuss recent progress on using single-molecule fluorescence methods for probing both intra- and intermolecular interactions of IDPs. We focus on several aspects, including models for analyzing experimental data, physical principles that can be obtained, and combinations with other experimental methods for a comprehensive view of the structural properties of IDPs.
Original language | English (US) |
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Title of host publication | Intrinsically Disordered Proteins |
Subtitle of host publication | Dynamics, Binding, and Function |
Publisher | Elsevier |
Pages | 93-136 |
Number of pages | 44 |
ISBN (Electronic) | 9780128163481 |
ISBN (Print) | 9780128167328 |
DOIs | |
State | Published - Jan 1 2019 |
Externally published | Yes |
Keywords
- Fluorescence correlation spectroscopy
- Molecule dynamics simulation
- Polymer model
- Protein dynamics
- Single-molecule Förster resonance energy transfer
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology