Single-molecule experiments in synthetic biology: An approach to the affinity ranking of DNA-binding peptides

Rainer Eckel; Sven David Wilking; Anke Becker; Norbert Sewald; Robert Ros; Dario Anselmetti

doi:10.1002/anie.200500152

Single-molecule experiments in synthetic biology: An approach to the affinity ranking of DNA-binding peptides

Rainer Eckel, Sven David Wilking, Anke Becker, Norbert Sewald, Robert Ros, Dario Anselmetti

Research output: Contribution to journal › Article › peer-review

53 Scopus citations

Abstract

(Figure Presented) Peptide-DNA recognition: The specific binding of peptides to DNA representing a section of the binding sequence of a transcription activator has been demonstrated for the first time at the single-molecule level with AFM (experimental principle, see picture). The results underscore the importance of peptide length and cooperative effects as well as the contribution of single-point mutations on specific binding.

Original language	English (US)
Pages (from-to)	3921-3924
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	44
Issue number	25
DOIs	https://doi.org/10.1002/anie.200500152
State	Published - Jun 20 2005
Externally published	Yes

Keywords

Molecular recognition
Peptides
Point mutations
Scanning probe microscopy
Single-molecule studies

ASJC Scopus subject areas

Catalysis
Chemistry(all)

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10.1002/anie.200500152

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abstract = "(Figure Presented) Peptide-DNA recognition: The specific binding of peptides to DNA representing a section of the binding sequence of a transcription activator has been demonstrated for the first time at the single-molecule level with AFM (experimental principle, see picture). The results underscore the importance of peptide length and cooperative effects as well as the contribution of single-point mutations on specific binding.",

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T2 - An approach to the affinity ranking of DNA-binding peptides

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AU - Wilking, Sven David

AU - Becker, Anke

AU - Sewald, Norbert

AU - Ros, Robert

AU - Anselmetti, Dario

PY - 2005/6/20

Y1 - 2005/6/20

N2 - (Figure Presented) Peptide-DNA recognition: The specific binding of peptides to DNA representing a section of the binding sequence of a transcription activator has been demonstrated for the first time at the single-molecule level with AFM (experimental principle, see picture). The results underscore the importance of peptide length and cooperative effects as well as the contribution of single-point mutations on specific binding.

AB - (Figure Presented) Peptide-DNA recognition: The specific binding of peptides to DNA representing a section of the binding sequence of a transcription activator has been demonstrated for the first time at the single-molecule level with AFM (experimental principle, see picture). The results underscore the importance of peptide length and cooperative effects as well as the contribution of single-point mutations on specific binding.

KW - Molecular recognition

KW - Peptides

KW - Point mutations

KW - Scanning probe microscopy

KW - Single-molecule studies

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Single-molecule experiments in synthetic biology: An approach to the affinity ranking of DNA-binding peptides

Abstract

Keywords

ASJC Scopus subject areas

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