Single-molecule experiments in synthetic biology: An approach to the affinity ranking of DNA-binding peptides

Rainer Eckel; Sven David Wilking; Anke Becker; Norbert Sewald; Robert Ros; Dario Anselmetti

doi:10.1002/anie.200500152

Single-molecule experiments in synthetic biology: An approach to the affinity ranking of DNA-binding peptides

Rainer Eckel, Sven David Wilking, Anke Becker, Norbert Sewald, Robert Ros, Dario Anselmetti

Research output: Contribution to journal › Article › peer-review

53 Scopus citations

Abstract

(Figure Presented) Peptide-DNA recognition: The specific binding of peptides to DNA representing a section of the binding sequence of a transcription activator has been demonstrated for the first time at the single-molecule level with AFM (experimental principle, see picture). The results underscore the importance of peptide length and cooperative effects as well as the contribution of single-point mutations on specific binding.

Original language	English (US)
Pages (from-to)	3921-3924
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	44
Issue number	25
DOIs	https://doi.org/10.1002/anie.200500152
State	Published - Jun 20 2005
Externally published	Yes

Keywords

Molecular recognition
Peptides
Point mutations
Scanning probe microscopy
Single-molecule studies

ASJC Scopus subject areas

Catalysis
General Chemistry

Access to Document

10.1002/anie.200500152

Cite this

@article{f14e153f565443b6b4eeb9d342160934,

title = "Single-molecule experiments in synthetic biology: An approach to the affinity ranking of DNA-binding peptides",

abstract = "(Figure Presented) Peptide-DNA recognition: The specific binding of peptides to DNA representing a section of the binding sequence of a transcription activator has been demonstrated for the first time at the single-molecule level with AFM (experimental principle, see picture). The results underscore the importance of peptide length and cooperative effects as well as the contribution of single-point mutations on specific binding.",

keywords = "Molecular recognition, Peptides, Point mutations, Scanning probe microscopy, Single-molecule studies",

author = "Rainer Eckel and Wilking, {Sven David} and Anke Becker and Norbert Sewald and Robert Ros and Dario Anselmetti",

year = "2005",

month = jun,

day = "20",

doi = "10.1002/anie.200500152",

language = "English (US)",

volume = "44",

pages = "3921--3924",

journal = "Angewandte Chemie - International Edition",

issn = "1433-7851",

publisher = "John Wiley and Sons Ltd",

number = "25",

}

TY - JOUR

T1 - Single-molecule experiments in synthetic biology

T2 - An approach to the affinity ranking of DNA-binding peptides

AU - Eckel, Rainer

AU - Wilking, Sven David

AU - Becker, Anke

AU - Sewald, Norbert

AU - Ros, Robert

AU - Anselmetti, Dario

PY - 2005/6/20

Y1 - 2005/6/20

N2 - (Figure Presented) Peptide-DNA recognition: The specific binding of peptides to DNA representing a section of the binding sequence of a transcription activator has been demonstrated for the first time at the single-molecule level with AFM (experimental principle, see picture). The results underscore the importance of peptide length and cooperative effects as well as the contribution of single-point mutations on specific binding.

AB - (Figure Presented) Peptide-DNA recognition: The specific binding of peptides to DNA representing a section of the binding sequence of a transcription activator has been demonstrated for the first time at the single-molecule level with AFM (experimental principle, see picture). The results underscore the importance of peptide length and cooperative effects as well as the contribution of single-point mutations on specific binding.

KW - Molecular recognition

KW - Peptides

KW - Point mutations

KW - Scanning probe microscopy

KW - Single-molecule studies

UR - http://www.scopus.com/inward/record.url?scp=21244479084&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=21244479084&partnerID=8YFLogxK

U2 - 10.1002/anie.200500152

DO - 10.1002/anie.200500152

M3 - Article

C2 - 15906400

AN - SCOPUS:21244479084

SN - 1433-7851

VL - 44

SP - 3921

EP - 3924

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 25

ER -

Single-molecule experiments in synthetic biology: An approach to the affinity ranking of DNA-binding peptides

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this