Silicon-based ion channel sensor

Michael Goryll; S. Wilk; G. M. Laws; Trevor Thornton; Stephen Goodnick; Marco Saraniti; J. Tang; R. S. Eisenberg

doi:10.1016/j.spmi.2004.03.041

Silicon-based ion channel sensor

Michael Goryll, S. Wilk, G. M. Laws, Trevor Thornton, Stephen Goodnick, Marco Saraniti, J. Tang, R. S. Eisenberg

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

In this paper we present a method to fabricate an aperture in a silicon wafer that can be used to suspend a freestanding lipid bilayer membrane. The design offers the feature of scalability of the aperture size into the submicron range. Lipid bilayer membranes formed across the aperture in the oxidized silicon substrate show a gigaohm sealing resistance. The stability of these membranes allowed the insertion of a nanometer-sized ion channel protein (OmpF porin) and the measurement of voltage dependent gating that can be expected from a working porin ion channel.

Original language	English (US)
Pages (from-to)	451-457
Number of pages	7
Journal	Superlattices and Microstructures
Volume	34
Issue number	3-6
DOIs	https://doi.org/10.1016/j.spmi.2004.03.041
State	Published - Sep 1 2003

Keywords

Biosensors
Lipid bilayers
Silicon devices

ASJC Scopus subject areas

Materials Science(all)
Condensed Matter Physics
Electrical and Electronic Engineering

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10.1016/j.spmi.2004.03.041

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abstract = "In this paper we present a method to fabricate an aperture in a silicon wafer that can be used to suspend a freestanding lipid bilayer membrane. The design offers the feature of scalability of the aperture size into the submicron range. Lipid bilayer membranes formed across the aperture in the oxidized silicon substrate show a gigaohm sealing resistance. The stability of these membranes allowed the insertion of a nanometer-sized ion channel protein (OmpF porin) and the measurement of voltage dependent gating that can be expected from a working porin ion channel.",

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AU - Goryll, Michael

AU - Wilk, S.

AU - Laws, G. M.

AU - Thornton, Trevor

AU - Goodnick, Stephen

AU - Saraniti, Marco

AU - Tang, J.

AU - Eisenberg, R. S.

PY - 2003/9/1

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N2 - In this paper we present a method to fabricate an aperture in a silicon wafer that can be used to suspend a freestanding lipid bilayer membrane. The design offers the feature of scalability of the aperture size into the submicron range. Lipid bilayer membranes formed across the aperture in the oxidized silicon substrate show a gigaohm sealing resistance. The stability of these membranes allowed the insertion of a nanometer-sized ion channel protein (OmpF porin) and the measurement of voltage dependent gating that can be expected from a working porin ion channel.

AB - In this paper we present a method to fabricate an aperture in a silicon wafer that can be used to suspend a freestanding lipid bilayer membrane. The design offers the feature of scalability of the aperture size into the submicron range. Lipid bilayer membranes formed across the aperture in the oxidized silicon substrate show a gigaohm sealing resistance. The stability of these membranes allowed the insertion of a nanometer-sized ion channel protein (OmpF porin) and the measurement of voltage dependent gating that can be expected from a working porin ion channel.

KW - Biosensors

KW - Lipid bilayers

KW - Silicon devices

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JO - Superlattices and Microstructures

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Silicon-based ion channel sensor

Abstract

Keywords

ASJC Scopus subject areas

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