Abstract
Crystal structure determination of biological macromolecules using the novel technique of serial femtosecond crystallography (SFX) is severely limited by the scarcity of X-ray free-electron laser (XFEL) sources. However, recent and future upgrades render microfocus beamlines at synchrotron-radiation sources suitable for room-temperature serial crystallography data collection also. Owing to the longer exposure times that are needed at synchrotrons, serial data collection is termed serial millisecond crystallography (SMX). As a result, the number of SMX experiments is growing rapidly, with a dozen experiments reported so far. Here, the first high-viscosity injector-based SMX experiments carried out at a US synchrotron source, the Advanced Photon Source (APS), are reported. Microcrystals (5-20μm) of a wide variety of proteins, including lysozyme, thaumatin, phycocyanin, the human A 2A adenosine receptor (A 2A AR), the soluble fragment of the membrane lipoprotein Flpp3 and proteinase K, were screened. Crystals suspended in lipidic cubic phase (LCP) or a high-molecular-weight poly(ethylene oxide) (PEO; molecular weight 8000000) were delivered to the beam using a high-viscosity injector. In-house data-reduction (hit-finding) software developed at APS as well as the SFX data-reduction and analysis software suites Cheetah and CrystFEL enabled efficient on-site SMX data monitoring, reduction and processing. Complete data sets were collected for A 2A AR, phycocyanin, Flpp3, proteinase K and lysozyme, and the structures of A 2A AR, phycocyanin, proteinase K and lysozyme were determined at 3.2, 3.1, 2.65 and 2.05Å resolution, respectively. The data demonstrate the feasibility of serial millisecond crystallography from 5-20μm crystals using a high-viscosity injector at APS. The resolution of the crystal structures obtained in this study was dictated by the current flux density and crystal size, but upcoming developments in beamline optics and the planned APS-U upgrade will increase the intensity by two orders of magnitude. These developments will enable structure determination from smaller and/or weakly diffracting microcrystals.
Original language | English (US) |
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Pages (from-to) | 439-454 |
Number of pages | 16 |
Journal | IUCrJ |
Volume | 4 |
DOIs | |
State | Published - 2017 |
Keywords
- Advanced Photon Source
- high-viscosity injector
- serial millisecond crystallography
- synchrotron radiation
ASJC Scopus subject areas
- General Chemistry
- Biochemistry
- General Materials Science
- Condensed Matter Physics
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Serial Millisecond Crystallography of Membrane and Soluble Protein Micro-crystals using Synchrotron Radiation
Martin-Garcia, J. M. (Contributor), Conrad, C. E. (Contributor), Nelson, G. (Contributor), Stander, N. (Contributor), Zatsepin, N. A. (Contributor), Zook, J. (Contributor), Zhu, L. (Contributor), Geiger, J. (Contributor), Chun, E. (Contributor), Kissick, D. (Contributor), Hilgart, M. C. (Contributor), Ogata, C. (Contributor), Ishchenko, A. (Contributor), Nagaratnam, N. (Contributor), Roy-Chowdhury, S. (Contributor), Coe, J. (Contributor), Subramanian, G. (Contributor), Schaffer, A. (Contributor), James, D. (Contributor), Ketwala, G. (Contributor), Venugopalan, N. (Contributor), Xu, S. (Contributor), Corcoran, S. (Contributor), Ferguson, D. (Contributor), Weierstall, U. (Contributor), Spence, J. C. H. (Contributor), Cherezov, V. (Contributor), Fromme, P. (Contributor), Fischetti, R. F. (Contributor) & Liu, W. (Contributor), Protein Data Bank (PDB), May 24 2017
DOI: 10.2210/pdb5UVK, https://www.wwpdb.org/pdb?id=pdb_00005uvk
Dataset
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Serial Millisecond Crystallography of Membrane and Soluble Protein Micro-crystals using Synchrotron Radiation
Martin-Garcia, J. M. (Contributor), Conrad, C. E. (Contributor), Nelson, G. (Contributor), Stander, N. (Contributor), Zatsepin, N. A. (Contributor), Zook, J. (Contributor), Zhu, L. (Contributor), Geiger, J. (Contributor), Chun, E. (Contributor), Kissick, D. (Contributor), Hilgart, M. C. (Contributor), Ogata, C. (Contributor), Ishchenko, A. (Contributor), Nagaratnam, N. (Contributor), Roy-Chowdhury, S. (Contributor), Coe, J. (Contributor), Subramanian, G. (Contributor), Schaffer, A. (Contributor), James, D. (Contributor), Ketwala, G. (Contributor), Venugopalan, N. (Contributor), Xu, S. (Contributor), Corcoran, S. (Contributor), Ferguson, D. (Contributor), Weierstall, U. (Contributor), Spence, J. C. H. (Contributor), Cherezov, V. (Contributor), Fromme, P. (Contributor), Fischetti, R. F. (Contributor) & Liu, W. (Contributor), Protein Data Bank (PDB), May 24 2017
DOI: 10.2210/pdb5UVL, https://www.wwpdb.org/pdb?id=pdb_00005uvl
Dataset
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Serial Millisecond Crystallography of Membrane and Soluble Protein Micro-crystals using Synchrotron Radiation
Martin-Garcia, J. M. (Contributor), Conrad, C. E. (Contributor), Nelson, G. (Contributor), Stander, N. (Contributor), Zatsepin, N. A. (Contributor), Zook, J. (Contributor), Zhu, L. (Contributor), Geiger, J. (Contributor), Chun, E. (Contributor), Kissick, D. (Contributor), Hilgart, M. C. (Contributor), Ogata, C. (Contributor), Ishchenko, A. (Contributor), Nagaratnam, N. (Contributor), Roy-Chowdhury, S. (Contributor), Coe, J. (Contributor), Subramanian, G. (Contributor), Schaffer, A. (Contributor), James, D. (Contributor), Ketwala, G. (Contributor), Venugopalan, N. (Contributor), Xu, S. (Contributor), Corcoran, S. (Contributor), Ferguson, D. (Contributor), Weierstall, U. (Contributor), Spence, J. C. H. (Contributor), Cherezov, V. (Contributor), Fromme, P. (Contributor), Fischetti, R. F. (Contributor) & Liu, W. (Contributor), Protein Data Bank (PDB), May 24 2017
DOI: 10.2210/pdb5UVI, https://www.wwpdb.org/pdb?id=pdb_00005uvi
Dataset