Abstract
The increase in successful adaptations of serial crystallography at synchrotron radiation sources continues. To date, the number of serial synchrotron crystallography (SSX) experiments has grown exponentially, with over 40 experiments reported so far. In this work, we report the first SSX experiments with viscous jets conducted at ALBA beamline BL13-XALOC. Small crystals (15-30 μm) of five soluble proteins (lysozyme, proteinase K, phycocyanin, insulin and α-spectrin-SH3 domain) were suspended in lipidic cubic phase (LCP) and delivered to the X-ray beam with a high-viscosity injector developed at Arizona State University. Complete data sets were collected from all proteins and their high-resolution structures determined. The high quality of the diffraction data collected from all five samples, and the lack of specific radiation damage in the structures obtained in this study, confirm that the current capabilities at the beamline enables atomic resolution determination of protein structures from microcrystals as small as 15 μm using viscous jets at room temperature. Thus, BL13-XALOC can provide a feasible alternative to X-ray free-electron lasers when determining snapshots of macromolecular structures.
Original language | English (US) |
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Pages (from-to) | 896-907 |
Number of pages | 12 |
Journal | Journal of synchrotron radiation |
Volume | 29 |
DOIs | |
State | Published - May 1 2022 |
Keywords
- ALBA
- LCP
- XALOC
- microcrystal
- serial synchrotron crystallography
- viscous jet
ASJC Scopus subject areas
- Radiation
- Nuclear and High Energy Physics
- Instrumentation
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Dive into the research topics of 'Serial macromolecular crystallography at ALBA Synchrotron Light Source'. Together they form a unique fingerprint.Datasets
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Serial Macromolecular Crystallography at ALBA Synchrotron Light Source - Insulin
Martin-Garcia, J. M. (Contributor), Botha, S. (Contributor), Hu, H. (Contributor), Jernigan, R. (Contributor), Castellví, A. (Contributor), Lisova, S. (Contributor), Gil, F. (Contributor), Calisto, B. (Contributor), Crespo, I. (Contributor), Roy-Chowdhury, S. (Contributor), Grieco, A. (Contributor), Ketawala, G. (Contributor), Weierstall, U. (Contributor), Spence, J. (Contributor), Fromme, P. (Contributor), Zatsepin, N. (Contributor), Boer, D. R. (Contributor) & Carpena, X. (Contributor), Protein Data Bank (PDB), Oct 27 2021
DOI: 10.2210/pdb7S4Y, https://www.wwpdb.org/pdb?id=pdb_00007s4y
Dataset
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Serial Macromolecular Crystallography at ALBA Synchrotron Light Source - Phycocyanin
Martin-Garcia, J. M. (Contributor), Botha, S. (Contributor), Hu, H. (Contributor), Jernigan, R. (Contributor), Castellví, A. (Contributor), Lisova, S. (Contributor), Gil, F. (Contributor), Calisto, B. (Contributor), Crespo, I. (Contributor), Roy-Chowdhury, S. (Contributor), Grieco, A. (Contributor), Ketawala, G. (Contributor), Weierstall, U. (Contributor), Spence, J. (Contributor), Fromme, P. (Contributor), Zatsepin, N. (Contributor), Boer, D. R. (Contributor) & Carpena, X. (Contributor), Protein Data Bank (PDB), Oct 27 2021
DOI: 10.2210/pdb7S50, https://www.wwpdb.org/pdb?id=pdb_00007s50
Dataset
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Serial Macromolecular Crystallography at ALBA Synchrotron Light Source - Proteinase K
Martin-Garcia, J. M. (Contributor), Botha, S. (Contributor), Hu, H. (Contributor), Jernigan, R. (Contributor), Castellví, A. (Contributor), Lisova, S. (Contributor), Gil, F. (Contributor), Calisto, B. (Contributor), Crespo, I. (Contributor), Roy-Chowdhury, S. (Contributor), Grieco, A. (Contributor), Ketawala, G. (Contributor), Weierstall, U. (Contributor), Spence, J. (Contributor), Fromme, P. (Contributor), Zatsepin, N. (Contributor), Boer, D. R. (Contributor) & Carpena, X. (Contributor), Protein Data Bank (PDB), Oct 27 2021
DOI: 10.2210/pdb7S4Z, https://www.wwpdb.org/pdb?id=pdb_00007s4z
Dataset