Sequential gene function in the initiation of Saccharomyces cerevisiae DNA synthesis

Lynna M. Hereford, L. H. Hartwell

Research output: Contribution to journalArticlepeer-review

169 Scopus citations


Four steps are known to be required for the initiation of DNA synthesis in Saccharomyces cerevisiae. Three of these are mediated by the products of genes cdc 4, 7, and 28 and the fourth is identified by the inhibition exerted on haploid α cells by the mating pheromone, α factor. These four steps have been ordered by a combination of two methods and found to be: {A figure is presented} initiation of DNA synthesis The two sequencing methods are described in detail. Experiments involving the shift of mutant cells from the restrictive to the permissive temperature in the presence of cycloheximide demonstrated that the protein synthesis requirement for yeast DNA replication can be completed before the cdc 7-mediated step.

Original languageEnglish (US)
Pages (from-to)445-456,IN13-IN14,457-461
JournalJournal of molecular biology
Issue number3
StatePublished - Apr 15 1974
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology


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