Sequence of Escherichia coli O138 O-antigen gene cluster and gne identification by bioinformatics

Qing Ke Kong, Jian Song Cheng, Guang Zhao, Lei Wang, Hong Jie Guo

Research output: Contribution to journalArticlepeer-review


E. coli O138 is one of the enterotoxigenic Escherichia coli, causing the postweaning diarrhea and edema disease of weaned pigs. The O-antigen gene cluster of E. coli O138 was sequenced and found to contain the genes rmlB-DAC and gne, gna for the biosynthesis of nucleotide sugars dTDP-rhamnose and UDP-GalNAcA, respectively, genes encoding for O unit flippase(wzx), O-antigen polymerase(wzy) and 3 potential transferase genes. The possible biosynthesis pathway for rare UDP-GalNAcA was proposed. Two genes specific to E. coli O138 were identified. This work provides the basis for a sensitive test by PCR for the rapid detection of E. coli O138. Phylogenetic tree for Gne and GalE proteins was generated and comparisons were made among different strains, and results revealed that these proteins are similar in the second structure, and Gne of E. coli O138 was identified by bioinformatics.

Original languageEnglish (US)
Pages (from-to)756-760
Number of pages5
JournalWei sheng wu xue bao = Acta microbiologica Sinica
Issue number6
StatePublished - Dec 2004
Externally publishedYes

ASJC Scopus subject areas

  • General Medicine


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