Separation of the bryostatin derivatives by high performance liquid chromatography

Yoshiaki Kamano; Ayano Kotake; Toshihiko Nogawa; Hatsue Hiraide; George Pettit; Cherry L. Herald

doi:10.1081/JLC-100101459

Separation of the bryostatin derivatives by high performance liquid chromatography

Yoshiaki Kamano, Ayano Kotake, Toshihiko Nogawa, Hatsue Hiraide, George Pettit, Cherry L. Herald

Molecular Sciences, School of (SMS)

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

The separation of bryostatin derivatives (26-acetate, 26- metabromobenzoate, 26-ketone, and 13→30 epoxide) by high performance liquid chromatography (HPLC) using normal phase and reverse phase systems was accomplished. Using n-hexane/EtOAc/MeOH/H₂O (26:5:1:0.01) as mobile phase for normal phase HPLC and acetonitrile (CH₃CN)/H₂O system for reverse phase HPLC provided good separation of bryostatin derivatives. In addition, contributions of functional group for eluate order were discussed.

Original language	English (US)
Pages (from-to)	399-409
Number of pages	11
Journal	Journal of Liquid Chromatography and Related Technologies
Volume	23
Issue number	3
DOIs	https://doi.org/10.1081/JLC-100101459
State	Published - 2000

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Pharmaceutical Science
Clinical Biochemistry

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abstract = "The separation of bryostatin derivatives (26-acetate, 26- metabromobenzoate, 26-ketone, and 13→30 epoxide) by high performance liquid chromatography (HPLC) using normal phase and reverse phase systems was accomplished. Using n-hexane/EtOAc/MeOH/H2O (26:5:1:0.01) as mobile phase for normal phase HPLC and acetonitrile (CH3CN)/H2O system for reverse phase HPLC provided good separation of bryostatin derivatives. In addition, contributions of functional group for eluate order were discussed.",

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T1 - Separation of the bryostatin derivatives by high performance liquid chromatography

AU - Kamano, Yoshiaki

AU - Kotake, Ayano

AU - Nogawa, Toshihiko

AU - Hiraide, Hatsue

AU - Pettit, George

AU - Herald, Cherry L.

PY - 2000

Y1 - 2000

N2 - The separation of bryostatin derivatives (26-acetate, 26- metabromobenzoate, 26-ketone, and 13→30 epoxide) by high performance liquid chromatography (HPLC) using normal phase and reverse phase systems was accomplished. Using n-hexane/EtOAc/MeOH/H2O (26:5:1:0.01) as mobile phase for normal phase HPLC and acetonitrile (CH3CN)/H2O system for reverse phase HPLC provided good separation of bryostatin derivatives. In addition, contributions of functional group for eluate order were discussed.

AB - The separation of bryostatin derivatives (26-acetate, 26- metabromobenzoate, 26-ketone, and 13→30 epoxide) by high performance liquid chromatography (HPLC) using normal phase and reverse phase systems was accomplished. Using n-hexane/EtOAc/MeOH/H2O (26:5:1:0.01) as mobile phase for normal phase HPLC and acetonitrile (CH3CN)/H2O system for reverse phase HPLC provided good separation of bryostatin derivatives. In addition, contributions of functional group for eluate order were discussed.

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Separation of the bryostatin derivatives by high performance liquid chromatography

Abstract

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