@article{ec39d1403a5c4a74bea14f52a453be10,
title = "Seminal fluid protein divergence among populations exhibiting postmating prezygotic reproductive isolation",
abstract = "Despite holding a central role in fertilization, reproductive traits often show elevated rates of evolution and diversification. The rapid evolution of seminal fluid proteins (Sfps) within populations is predicted to cause mis-signalling between the male ejaculate and the female during and after mating resulting in postmating prezygotic (PMPZ) isolation between populations. Crosses between Drosophila montana populations show PMPZ isolation in the form of reduced fertilization success in both noncompetitive and competitive contexts. Here we test whether male ejaculate proteins produced in the accessory glands or ejaculatory bulb differ between populations using liquid chromatography tandem mass spectrometry. We find more than 150 differentially abundant proteins between populations that may contribute to PMPZ isolation, including a number of proteases, peptidases and several orthologues of Drosophila melanogaster Sfps known to mediate fertilization success. Males from the population that elicit the stronger PMPZ isolation after mating with foreign females typically produced greater quantities of Sfps. The accessory glands and ejaculatory bulb show enrichment for different gene ontology (GO) terms and the ejaculatory bulb contributes more differentially abundant proteins. Proteins with a predicted secretory signal evolve faster than nonsecretory proteins. Finally, we take advantage of quantitative proteomics data for three Drosophila species to determine shared and unique GO enrichments of Sfps between taxa and which potentially mediate PMPZ isolation. Our study provides the first high-throughput quantitative proteomic evidence showing divergence of reproductive proteins between populations that exhibit PMPZ isolation.",
keywords = "Drosophila, accessory glands, ejaculate, postcopulatory sexual selection, postmating prezygotic isolation, proteomics, reproductive isolation, seminal fluid proteins, speciation, tandem mass-spectrometry",
author = "Garlovsky, {Martin D.} and Caroline Evans and Rosenow, {Mathew A.} and Karr, {Timothy L.} and Snook, {Rhonda R.}",
note = "Funding Information: Thanks go to Priyanka Prajapati and Alexander Charles for assistance with proteomics data analysis, and Irem Sepil and the organizers and attendees of the Joint Wellcome Trust/EMBL-EBI Proteomics Bioinformatics course 2017 for discussion about analyses. Alison Wright, Daniela Palmer, Leeban Yusuf, Henry Barton and Toni Gossman provided helpful discussion and scripts for performing evolutionary rates analysis. We are grateful to Anneli Hoikkala for providing fly stocks, Mike Ritchie and Darren Parker for access to genomic and proteomic resources, and Roger Butlin for valuable feedback throughout the project. M.D.G. was able to attend the Wellcome Trust/EMBL-EBI Proteomics Bioinformatics course thanks to a University of Sheffield Postgraduate Research Experience Programme (PREP) grant and was supported by the Adapting to the Challenges of a Changing Environment (ACCE) Doctoral Training Partnership grant NE/L002450/1, funded by the Natural Environment Research Council (NERC). Costs for proteomics were funded by a Royal Society Leverhulme Trust Senior Research Fellowship to R.R.S. C.E. acknowledges financial support from the Engineering and Physical Sciences Research Council, the ChELSI initiative (EP/E036252/1). Three anonymous reviewers provided constructive comments which improved the manuscript. Funding Information: Thanks go to Priyanka Prajapati and Alexander Charles for assistance with proteomics data analysis, and Irem Sepil and the organizers and attendees of the Joint Wellcome Trust/EMBL‐EBI Proteomics Bioinformatics course 2017 for discussion about analyses. Alison Wright, Daniela Palmer, Leeban Yusuf, Henry Barton and Toni Gossman provided helpful discussion and scripts for performing evolutionary rates analysis. We are grateful to Anneli Hoikkala for providing fly stocks, Mike Ritchie and Darren Parker for access to genomic and proteomic resources, and Roger Butlin for valuable feedback throughout the project. M.D.G. was able to attend the Wellcome Trust/EMBL‐EBI Proteomics Bioinformatics course thanks to a University of Sheffield Postgraduate Research Experience Programme (PREP) grant and was supported by the Adapting to the Challenges of a Changing Environment (ACCE) Doctoral Training Partnership grant NE/L002450/1, funded by the Natural Environment Research Council (NERC). Costs for proteomics were funded by a Royal Society Leverhulme Trust Senior Research Fellowship to R.R.S. C.E. acknowledges financial support from the Engineering and Physical Sciences Research Council, the ChELSI initiative (EP/E036252/1). Three anonymous reviewers provided constructive comments which improved the manuscript. Publisher Copyright: {\textcopyright} 2020 The Authors. Molecular Ecology published by John Wiley & Sons Ltd",
year = "2020",
month = nov,
day = "1",
doi = "10.1111/mec.15636",
language = "English (US)",
volume = "29",
pages = "4428--4441",
journal = "Molecular ecology",
issn = "0962-1083",
publisher = "Wiley-Blackwell",
number = "22",
}