TY - JOUR
T1 - Self-Assembly of Hybrid Peptide−DNA Nanostructures using Homotrimeric Coiled-Coil/Nucleic Acid Building Blocks
AU - Buchberger, Alex
AU - Al-Amin, Md
AU - Simmons, Chad R.
AU - Stephanopoulos, Nicholas
N1 - Publisher Copyright:
© 2023 Wiley-VCH GmbH.
PY - 2023/9/1
Y1 - 2023/9/1
N2 - Peptides and DNA are two of the most commonly used self-assembling biological molecules for the construction of nanomaterials. However, there are only a few examples that combine these two self-assembly motifs as key structural elements in a nanostructure. We report on the synthesis of a peptide−DNA conjugate that self-assembles into a stable homotrimer based on the coiled-coil motif. The hybrid peptide−DNA trimer was then used as a novel three-way junction to link together either small DNA tile nanostructures, or to close up a triangular wireframe DNA structure. The resulting nanostructures were characterized by atomic force microscopy, and compared with a scrambled, non-assembling peptide as a control. These hybrid nanostructures enable the integration of peptide motifs and potentially bio-functionality with DNA nanostructures, and open the door to novel nano-materials that have the advantages of both molecules.
AB - Peptides and DNA are two of the most commonly used self-assembling biological molecules for the construction of nanomaterials. However, there are only a few examples that combine these two self-assembly motifs as key structural elements in a nanostructure. We report on the synthesis of a peptide−DNA conjugate that self-assembles into a stable homotrimer based on the coiled-coil motif. The hybrid peptide−DNA trimer was then used as a novel three-way junction to link together either small DNA tile nanostructures, or to close up a triangular wireframe DNA structure. The resulting nanostructures were characterized by atomic force microscopy, and compared with a scrambled, non-assembling peptide as a control. These hybrid nanostructures enable the integration of peptide motifs and potentially bio-functionality with DNA nanostructures, and open the door to novel nano-materials that have the advantages of both molecules.
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U2 - 10.1002/cbic.202300223
DO - 10.1002/cbic.202300223
M3 - Article
C2 - 37099451
AN - SCOPUS:85158895041
SN - 1439-4227
VL - 24
JO - ChemBioChem
JF - ChemBioChem
IS - 17
M1 - e202300223
ER -