TY - GEN
T1 - Sample preparation in glass capillaries for high-throughput biochemical analyses
AU - Meldrum, Deirdre R.
AU - Holl, Mark R.
AU - Fisher, Charles H.
AU - Saini, Mohan S.
AU - McGuire, Shawn K.
AU - Ren, Timothy T.H.
AU - Pence, William H.
AU - Moody, Stephen E.
AU - Cunningham, David L.
AU - Donaldson, Douglas A.
AU - Wiktor, Peter J.
PY - 2005
Y1 - 2005
N2 - A capillary-based fluid handling system, the ACAPELLA-5K (A5K), has been developed to prepare precise mixtures of sample and reagents in glass capillaries at a rate of 5000 preparations in 8 hours. Following a user-defined protocol the system architecture enables sample aspiration, reagent dispensing, mixing, and thermal cycling, with intermediate imaging steps for in-process monitoring of critical fluid delivery steps. A serial pipeline process is used to provide flexibility, reproducibility, and reliability for the reactions prepared. A typical reaction comprises a 0.5 μL sample aspirated from a microplate well followed by 1 to 8 reagents dispensed in 40-100 pL droplet volumes using piezoelectric reagent dispensers. Reactions are then mixed to prepare a 2.0 μL final reaction volume. Reaction volumes from 0.5-2 μL have been demonstrated, representing performance comparable to the state-of-the-art in a majority of core sequencing facilities. A5K has been extensively tested in the preparation of deoxyribonucleic acid (DNA) polymerase chain reaction (PCR) and DNA sequencing reactions. Other applications of the A5K platform of technologies include quantitation of minimal residual disease, protein crystallography, and potential for application in drug discovery, forensics, and DNA analysis of environmental samples.
AB - A capillary-based fluid handling system, the ACAPELLA-5K (A5K), has been developed to prepare precise mixtures of sample and reagents in glass capillaries at a rate of 5000 preparations in 8 hours. Following a user-defined protocol the system architecture enables sample aspiration, reagent dispensing, mixing, and thermal cycling, with intermediate imaging steps for in-process monitoring of critical fluid delivery steps. A serial pipeline process is used to provide flexibility, reproducibility, and reliability for the reactions prepared. A typical reaction comprises a 0.5 μL sample aspirated from a microplate well followed by 1 to 8 reagents dispensed in 40-100 pL droplet volumes using piezoelectric reagent dispensers. Reactions are then mixed to prepare a 2.0 μL final reaction volume. Reaction volumes from 0.5-2 μL have been demonstrated, representing performance comparable to the state-of-the-art in a majority of core sequencing facilities. A5K has been extensively tested in the preparation of deoxyribonucleic acid (DNA) polymerase chain reaction (PCR) and DNA sequencing reactions. Other applications of the A5K platform of technologies include quantitation of minimal residual disease, protein crystallography, and potential for application in drug discovery, forensics, and DNA analysis of environmental samples.
KW - Biomechatronics
KW - Laboratory automation
KW - Microfluidics
KW - Sample preparation
UR - http://www.scopus.com/inward/record.url?scp=33745954752&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33745954752&partnerID=8YFLogxK
U2 - 10.1109/COASE.2005.1506737
DO - 10.1109/COASE.2005.1506737
M3 - Conference contribution
AN - SCOPUS:33745954752
SN - 0780394267
SN - 9780780394261
T3 - Proceedings of the 2005 IEEE Conference on Automation Science and Engineering, IEEE-CASE 2005
SP - 7
EP - 12
BT - Proceedings of the 2005 IEEE Conference on Automation Science and Engineering, IEEE-CASE 2005
T2 - 2005 IEEE Conference on Automation Science and Engineering, IEEE-CASE 2005
Y2 - 1 August 2005 through 2 August 2005
ER -