TY - JOUR
T1 - Salt-Dependent Conformational Changes of Intrinsically Disordered Proteins
AU - Wohl, Samuel
AU - Jakubowski, Matthew
AU - Zheng, Wenwei
N1 - Funding Information:
This work was supported by the National Science Foundation, Grant MCB-2015030, and the National Institutes of Health, Grant R01GM120537. The authors acknowledge Research Computing at Arizona State University for providing HPC and storage resources.
Publisher Copyright:
© 2021 American Chemical Society
PY - 2021/7/22
Y1 - 2021/7/22
N2 - The flexible structure of an intrinsically disordered protein (IDP) is known to be perturbed by salt concentrations, which can be understood by electrostatic screening on charged amino acids. However, an IDP usually contains more uncharged residues that are influenced by the salting-out effect. Here we have parametrized the salting-out effect into a coarse-grained model using a set of Förster resonance energy transfer data and verified with experimental salt-dependent liquid-liquid phase separation (LLPS) of 17 proteins. The new model can correctly capture the behavior of 6 more sequences, resulting in a total of 13 when varying salt concentrations. Together with a survey of more than 500 IDP sequences, we conclude that the salting-out effect, which was considered to be secondary to electrostatic screening, is important for IDP sequences with moderately charged residues at physiological salt concentrations. The presented scheme is generally applicable to other computational models for capturing salt-dependent IDP conformations.
AB - The flexible structure of an intrinsically disordered protein (IDP) is known to be perturbed by salt concentrations, which can be understood by electrostatic screening on charged amino acids. However, an IDP usually contains more uncharged residues that are influenced by the salting-out effect. Here we have parametrized the salting-out effect into a coarse-grained model using a set of Förster resonance energy transfer data and verified with experimental salt-dependent liquid-liquid phase separation (LLPS) of 17 proteins. The new model can correctly capture the behavior of 6 more sequences, resulting in a total of 13 when varying salt concentrations. Together with a survey of more than 500 IDP sequences, we conclude that the salting-out effect, which was considered to be secondary to electrostatic screening, is important for IDP sequences with moderately charged residues at physiological salt concentrations. The presented scheme is generally applicable to other computational models for capturing salt-dependent IDP conformations.
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U2 - 10.1021/acs.jpclett.1c01607
DO - 10.1021/acs.jpclett.1c01607
M3 - Article
C2 - 34259536
AN - SCOPUS:85111215861
SN - 1948-7185
VL - 12
SP - 6684
EP - 6691
JO - Journal of Physical Chemistry Letters
JF - Journal of Physical Chemistry Letters
IS - 28
ER -