Rotation of single live mammalian cells using dynamic holographic optical tweezers

Cao Bin Cao, Laimonas Kelbauskas, Samantha Chan, Rishabh M. Shetty, Dean Smith, Deirdre Meldrum

Research output: Contribution to journalArticlepeer-review

35 Scopus citations


We report on a method for rotating single mammalian cells about an axis perpendicular to the optical system axis through the imaging plane using dynamic holographic optical tweezers (HOTs). Two optical traps are created on the opposite edges of a mammalian cell and are continuously transitioned through the imaging plane along the circumference of the cell in opposite directions, thus providing the torque to rotate the cell in a controlled fashion. The method enables a complete 360° rotation of live single mammalian cells with spherical or near-to spherical shape in 3D space, and represents a useful tool suitable for the single cell analysis field, including tomographic imaging.

Original languageEnglish (US)
Pages (from-to)70-75
Number of pages6
JournalOptics and Lasers in Engineering
StatePublished - May 1 2017


  • Laser trapping
  • Optical manipulation
  • Optical tweezers
  • Single cells rotation
  • Spatial light modulation

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Mechanical Engineering
  • Electrical and Electronic Engineering


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