Restriction endonuclease analysis of Staphylococcus aureus plasmid DNA from three continents

B. N. Doebbeling, M. A. Pfaller, R. J. Hollis, L. D. Boyken, A. C. Pignatari, L. A. Herwaldt, R. P. Wenzel

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


Staphylococcus aureus isolates (n=1201) from 20 centers in Europe, the USA and Brazil were evaluated for the presence of epidemiologic markers. Plasmid typing and restriction endonuclease analysis of plasmid DNA confirmed the presence of an apparently identical plasmid in 13 % of clinical isolates. The plasmid was recovered from all 20 hospitals studied, with an overall frequency of >10 % on each of the three continents. Since relatively few staphylococcal plasmids may be shared by epidemiologically unrelated strains, there are inherent limitations to this otherwise useful technique. Additionally, these data demonstrate the importance of including unrelated strains of Staphylococcus aureus from the local region as controls when molecular typing methods are performed.

Original languageEnglish (US)
Pages (from-to)4-8
Number of pages5
JournalEuropean Journal of Clinical Microbiology & Infectious Diseases
Issue number1
StatePublished - Jan 1992
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases


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