@article{d9fcbbd9c14741c9b5a894e9fac0ce46,
title = "Resolution extension by image summing in serial femtosecond crystallography of two-dimensional membrane-protein crystals",
abstract = "Previous proof-of-concept measurements on single-layer two-dimensional membrane-protein crystals performed at X-ray free-electron lasers (FELs) have demonstrated that the collection of meaningful diffraction patterns, which is not possible at synchrotrons because of radiation-damage issues, is feasible. Here, the results obtained from the analysis of a thousand single-shot, room-temperature X-ray FEL diffraction images from two-dimensional crystals of a bacteriorhodopsin mutant are reported in detail. The high redundancy in the measurements boosts the intensity signal-to-noise ratio, so that the values of the diffracted intensities can be reliably determined down to the detector-edge resolution of 4'{\AA}. The results show that two-dimensional serial crystallography at X-ray FELs is a suitable method to study membrane proteins to near-atomic length scales at ambient temperature. The method presented here can be extended to pump-probe studies of optically triggered structural changes on submillisecond timescales in two-dimensional crystals, which allow functionally relevant large-scale motions that may be quenched in three-dimensional crystals.",
keywords = "free-electron lasers, membrane proteins, serial crystallography, two-dimensional crystals",
author = "Casadei, {Cecilia M.} and Tsai, {Ching Ju} and Anton Barty and Hunter, {Mark S.} and Nadia Zatsepin and Celestino Padeste and Guido Capitani and Benner, {W. Henry} and S{\'e}bastien Boutet and Hau-Riege, {Stefan P.} and Christopher Kupitz and Marc Messerschmidt and Ogren, {John I.} and Tom Pardini and Rothschild, {Kenneth J.} and Leonardo Sala and Brent Segelke and Williams, {Garth J.} and Evans, {James E.} and Li, {Xiao Dan} and Matthew Coleman and Bill Pedrini and Matthias Frank",
note = "Funding Information: This work was performed, in part, under the auspices of the US Department of Energy by Lawrence Livermore National Laboratory under contract DE-AC52-07NA27344 and was supported by LLNL Laboratory-Directed Research and Development (LDRD) project 12-ERD-031 and NIH grant 1R01GM117342-01. This work was supported in part by National Science Foundation grants (CBET-1264434 to KJR). Funding Information: This work was performed, in part, under the auspices of the US Department of Energy by Lawrence Livermore National Laboratory under contract DE-AC52-07NA27344 and was supported by LLNL Laboratory-Directed Research and Development (LDRD) project 12-ERD-031 and NIH grant 1R01GM117342-01. This work was supported in part by National Science Foundation grants (CBET-1264434 to KJR, including travel funding to JIO, 1231306 to MM, 1565180 and NSF-STC {\textquoteleft}BioXFEL{\textquoteright} award STC-1231306 to NAZ). Use of the Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory is supported by the US Department of Energy, Office of Science, Office of Basic Energy Sciences under contract No. DE-AC02-76SF00515. Publisher Copyright: {\textcopyright} Cecilia M. Casadei et al. 2018.",
year = "2018",
doi = "10.1107/S2052252517017043",
language = "English (US)",
volume = "5",
pages = "103--117",
journal = "IUCrJ",
issn = "2052-2525",
publisher = "International Union of Crystallography",
}