TY - JOUR
T1 - Refined procedure of evaluating experimental single-molecule force spectroscopy data
AU - Fuhrmann, Alexander
AU - Anselmetti, Dario
AU - Ros, Robert
AU - Getfert, Sebastian
AU - Reimann, Peter
PY - 2008/3/13
Y1 - 2008/3/13
N2 - Dynamic force spectroscopy is a well-established tool to study molecular recognition in a wide range of binding affinities on the single-molecule level. The theoretical interpretation of these data is still very challenging and the models describe the experimental data only partly. In this paper we reconsider the basic assumptions of the models on the basis of an experimental data set and propose an approach of analyzing and quantitatively evaluating dynamic force spectroscopy data on single ligand-receptor complexes. We present our procedure to process and analyze the force-distance curves, to detect the rupture events in an automated manner, and to calculate quantitative parameters for a biophysical characterization of the investigated interaction.
AB - Dynamic force spectroscopy is a well-established tool to study molecular recognition in a wide range of binding affinities on the single-molecule level. The theoretical interpretation of these data is still very challenging and the models describe the experimental data only partly. In this paper we reconsider the basic assumptions of the models on the basis of an experimental data set and propose an approach of analyzing and quantitatively evaluating dynamic force spectroscopy data on single ligand-receptor complexes. We present our procedure to process and analyze the force-distance curves, to detect the rupture events in an automated manner, and to calculate quantitative parameters for a biophysical characterization of the investigated interaction.
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U2 - 10.1103/PhysRevE.77.031912
DO - 10.1103/PhysRevE.77.031912
M3 - Article
C2 - 18517427
AN - SCOPUS:40949154233
SN - 1539-3755
VL - 77
JO - Physical Review E - Statistical, Nonlinear, and Soft Matter Physics
JF - Physical Review E - Statistical, Nonlinear, and Soft Matter Physics
IS - 3
M1 - 031912
ER -