TY - JOUR
T1 - Red-Shifted Coumarin Luciferins for Improved Bioluminescence Imaging
AU - Love, Anna C.
AU - Caldwell, Donald R.
AU - Kolbaba-Kartchner, Bethany
AU - Townsend, Katherine M.
AU - Halbers, Lila P.
AU - Yao, Zi
AU - Brennan, Caroline K.
AU - Ivanic, Joseph
AU - Hadjian, Tanya
AU - Mills, Jeremy H.
AU - Schnermann, Martin J.
AU - Prescher, Jennifer A.
N1 - Funding Information:
This work was supported by the U.S. National Institutes of Health (R01 GM107630 to J.A.P.) and the Intramural Research Program of the National Institutes of Health (NIH), NCI-CCR. This project has been further supported with Federal funds from the National Cancer Institute, National Institutes of Health, under Contract No. HHSN261200800001E. A.C.L. was supported by the National Science Foundation via the BEST IGERT (DGE-1144901) program. The authors thank the Barbara Karamos Cancer Center for contributing the 4T1, 4T07, and 66Cl4 cancer cell lines, and the NIH NCI-CCR for contributing the JIMT-1 cells. Some experiments were performed at the Laser Spectroscopy labs (LSL) at UCI. The authors thank Dr. Joseph Barchi, NCI-CCR, for NMR assistance and Dr. James Kelley, NCI-CCR, for mass spectrometric analysis. The authors also thank Dr. Jennifer Atwood for performing cell sorting on generated stable cell lines. They acknowledge the support of the Chao Family Comprehensive Cancer Center IFI Flow Cytometry Core Shared Resource, supported by the National Cancer Institute of the National Institutes of Health under award number P30CA062203. Additional thanks to the members of the Prescher lab for helpful discussions, along with members of the Weiss and Martin laboratories for providing equipment and reagents.
Publisher Copyright:
© 2023 American Chemical Society.
PY - 2023/2/15
Y1 - 2023/2/15
N2 - Multicomponent bioluminescence imaging in vivo requires an expanded collection of tissue-penetrant probes. Toward this end, we generated a new class of near-infrared (NIR) emitting coumarin luciferin analogues (CouLuc-3s). The scaffolds were easily accessed from commercially available dyes. Complementary mutant luciferases for the CouLuc-3 analogues were also identified. The brightest probes enabled sensitive imaging in vivo. The CouLuc-3 scaffolds are also orthogonal to popular bioluminescent reporters and can be used for multicomponent imaging applications. Collectively, this work showcases a new set of bioluminescent tools that can be readily implemented for multiplexed imaging in a variety of biological settings.
AB - Multicomponent bioluminescence imaging in vivo requires an expanded collection of tissue-penetrant probes. Toward this end, we generated a new class of near-infrared (NIR) emitting coumarin luciferin analogues (CouLuc-3s). The scaffolds were easily accessed from commercially available dyes. Complementary mutant luciferases for the CouLuc-3 analogues were also identified. The brightest probes enabled sensitive imaging in vivo. The CouLuc-3 scaffolds are also orthogonal to popular bioluminescent reporters and can be used for multicomponent imaging applications. Collectively, this work showcases a new set of bioluminescent tools that can be readily implemented for multiplexed imaging in a variety of biological settings.
UR - http://www.scopus.com/inward/record.url?scp=85148008173&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85148008173&partnerID=8YFLogxK
U2 - 10.1021/jacs.2c07220
DO - 10.1021/jacs.2c07220
M3 - Article
C2 - 36745536
AN - SCOPUS:85148008173
SN - 0002-7863
VL - 145
SP - 3335
EP - 3345
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
IS - 6
ER -