Abstract
We have used a DNA-aptamer tethered to an atomic force microscope probe to carry out recognition imaging of IgE molecules attached to a mica substrate. The recognition was efficient (∼90%) and specific, being blocked by injection of IgE molecules in solution, and not being interfered with by high concentrations of a second protein. The signal/noise ratio of the recognition signal was better than that obtained with antibodies, despite the fact that the average force required to break the aptamer-protein bonds was somewhat smaller.
Original language | English (US) |
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Pages (from-to) | 4236-4238 |
Number of pages | 3 |
Journal | Biophysical journal |
Volume | 90 |
Issue number | 11 |
DOIs | |
State | Published - Jun 2006 |
ASJC Scopus subject areas
- Biophysics