Rapid nonlinear image scanning microscopy

Ingo Gregor, Martin Spiecker, Roman Petrovsky, Jörg Großhans, Robert Ros, Jörg Enderlein

Research output: Contribution to journalArticlepeer-review

57 Scopus citations


Image scanning microscopy (ISM) doubles the resolution of a conventional confocal microscope for super-resolution imaging. Here, we describe an all-optical ISM design based on rescanning microscopy for two-photon-excited fluorescence and second-harmonic generation that allows straightforward implementation into existing microscopes. The design offers improved sensitivity and high frame rates relative to those of existing systems. We demonstrate its utility using fixed and living specimens as well as collagen hydrogels.

Original languageEnglish (US)
Pages (from-to)1087-1089
Number of pages3
JournalNature Methods
Issue number11
StatePublished - Oct 31 2017

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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