Rapid apolipoprotein phenotyping using mass spectrometric immunoassay approaches

Eric E. Niederkofler; Dobrin Nedelkov; Kemmons A. Tubbs; Urban A. Kiernan; Peter Williams; Randall W. Nelson

Rapid apolipoprotein phenotyping using mass spectrometric immunoassay approaches

Eric E. Niederkofler, Dobrin Nedelkov, Kemmons A. Tubbs, Urban A. Kiernan, Peter Williams, Randall W. Nelson

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Mass spectrometric immunoassay approaches were used for rapid apolipoprotein phenotyping. Affinity pipettes were prepared by immobilizing a specific anti-apolipoprotein antibody onto a small-volume porous frit fitted at the entrance of a wide bore P200 pipette tip. The samples were incubated against the affinity pipettes by repetitively drawing the samples through the pipettes. The proteins captured in the affinity pipettes were eluted onto MALDI-TOF mass spectrometry (MS) targets using acidified MALDI matrix solution.

Original language	English (US)
Title of host publication	Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages	253-254
Number of pages	2
State	Published - 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

ASJC Scopus subject areas

Spectroscopy

Cite this

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title = "Rapid apolipoprotein phenotyping using mass spectrometric immunoassay approaches",

abstract = "Mass spectrometric immunoassay approaches were used for rapid apolipoprotein phenotyping. Affinity pipettes were prepared by immobilizing a specific anti-apolipoprotein antibody onto a small-volume porous frit fitted at the entrance of a wide bore P200 pipette tip. The samples were incubated against the affinity pipettes by repetitively drawing the samples through the pipettes. The proteins captured in the affinity pipettes were eluted onto MALDI-TOF mass spectrometry (MS) targets using acidified MALDI matrix solution.",

author = "Niederkofler, {Eric E.} and Dobrin Nedelkov and Tubbs, {Kemmons A.} and Kiernan, {Urban A.} and Peter Williams and Nelson, {Randall W.}",

year = "2002",

language = "English (US)",

pages = "253--254",

booktitle = "Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

TY - GEN

T1 - Rapid apolipoprotein phenotyping using mass spectrometric immunoassay approaches

AU - Niederkofler, Eric E.

AU - Nedelkov, Dobrin

AU - Tubbs, Kemmons A.

AU - Kiernan, Urban A.

AU - Williams, Peter

AU - Nelson, Randall W.

PY - 2002

Y1 - 2002

N2 - Mass spectrometric immunoassay approaches were used for rapid apolipoprotein phenotyping. Affinity pipettes were prepared by immobilizing a specific anti-apolipoprotein antibody onto a small-volume porous frit fitted at the entrance of a wide bore P200 pipette tip. The samples were incubated against the affinity pipettes by repetitively drawing the samples through the pipettes. The proteins captured in the affinity pipettes were eluted onto MALDI-TOF mass spectrometry (MS) targets using acidified MALDI matrix solution.

AB - Mass spectrometric immunoassay approaches were used for rapid apolipoprotein phenotyping. Affinity pipettes were prepared by immobilizing a specific anti-apolipoprotein antibody onto a small-volume porous frit fitted at the entrance of a wide bore P200 pipette tip. The samples were incubated against the affinity pipettes by repetitively drawing the samples through the pipettes. The proteins captured in the affinity pipettes were eluted onto MALDI-TOF mass spectrometry (MS) targets using acidified MALDI matrix solution.

UR - http://www.scopus.com/inward/record.url?scp=2442715331&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2442715331&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:2442715331

SP - 253

EP - 254

BT - Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Rapid apolipoprotein phenotyping using mass spectrometric immunoassay approaches

Abstract

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ASJC Scopus subject areas

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