Abstract
Cancer cell motility and its heterogeneity play an important role in metastasis, which is responsible for death of 90% of cancer patients. Here, in combination with a microfluidic technique, single-cell tracking, and systematic motility analysis, we present a rapid and quantitative approach to judge the motility heterogeneity of breast cancer cells MDA-MB-231 and MCF-7 in a well-defined three-dimensional (3D) microenvironment with controllable conditions. Following this approach, identification of highly mobile active cells in a medium with epithelial growth factor will provide a practical tool for cell invasion and metastasis investigation of multiple cancer cell types, including primary cells. Further, this approach could potentially become a speedy (∼hours) and efficient tool for basic and clinical diagnosis.
| Original language | English (US) |
|---|---|
| Article number | 108701 |
| Journal | Chinese Physics B |
| Volume | 28 |
| Issue number | 10 |
| DOIs | |
| State | Published - 2019 |
Keywords
- cancer metastasis
- invasion potential
- motility heterogeneity
- three-dimensional microenvironment
ASJC Scopus subject areas
- Physics and Astronomy(all)
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In: Chinese Physics B, Vol. 28, No. 10, 108701, 2019.
Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - Quantitative heterogeneity and subgroup classification based on motility of breast cancer cells
AU - Xiong, Ling
AU - Liu, Yanping
AU - Liu, Ruchuan
AU - Yuan, Wei
AU - Wang, Gao
AU - He, Yi
AU - Shuai, Jianwei
AU - Jiao, Yang
AU - Zhang, Xixiang
AU - Han, Weijing
AU - Qu, Junle
AU - Liu, Liyu
N1 - Funding Information: Ling Xiong Yanping Liu Ruchuan Liu Wei Yuan Gao Wang Yi He Jianwei Shuai Yang Jiao Xixiang Zhang Weijing Han Junle Qu Liyu Liu Ling Xiong Yanping Liu Ruchuan Liu Wei Yuan Gao Wang Yi He Jianwei Shuai Yang Jiao Xixiang Zhang Weijing Han Junle Qu Liyu Liu Chongqing Key Laboratory of Soft Condensed Matter Physics and Smart Materials, College of Physics, Chongqing University, Chongqing 401331, China Department of Physics, Xiamen University, Xiamen 361005, China Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, China Materials Science and Engineering, Arizona State University, Tempe, Arizona 85287, USA Division of Physical Science and Engineering, King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia Shenzhen Shengyuan Biotechnology Co., Ltd, Shenzhen 518060, China Ling Xiong (熊玲), Yanping Liu (刘艳平), Ruchuan Liu (刘如川), Wei Yuan (袁伟), Gao Wang (王高), Yi He (何益), Jianwei Shuai (帅建伟), Yang Jiao (焦阳), Xixiang Zhang (张溪祥), Weijing Han (韩伟静), Junle Qu (屈军乐) and Liyu Liu (刘雳宇) 2019-09-01 2019-09-27 15:09:07 insert subject codes:domex_pacs cgi/release: Article released bin/incoming: New from .zip Cancer cell motility and its heterogeneity play an important role in metastasis, which is responsible for death of 90% of cancer patients. Here, in combination with a microfluidic technique, single-cell tracking, and systematic motility analysis, we present a rapid and quantitative approach to judge the motility heterogeneity of breast cancer cells MDA-MB-231 and MCF-7 in a well-defined three-dimensional (3D) microenvironment with controllable conditions. Following this approach, identification of highly mobile active cells in a medium with epithelial growth factor will provide a practical tool for cell invasion and metastasis investigation of multiple cancer cell types, including primary cells. Further, this approach could potentially become a speedy (∼hours) and efficient tool for basic and clinical diagnosis. � 2019 Chinese Physical Society and IOP Publishing Ltd [1] Sleeman J and Steeg P S 2010 Eur. J. Cancer 46 1177 https://doi.org/10.1016/j.ejca.2010.02.039 10.1016/j.ejca.2010.02.039 Sleeman J and Steeg P S Eur. J. Cancer 0959-8049 46 7 2010 1177 [2] Steeg P S and Theodorescu D 2008 Nat. Clin. Pract. 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Subjects 1840 2386 https://doi.org/10.1016/j.bbagen.2014.03.020 10.1016/j.bbagen.2014.03.020 Haeger A, Krause M, Wolf K and Friedl P Bba-Gen. Subjects 0304-4165 1840 8 2014 2386 Funding Information: Project supported by the National Natural Science Foundation of China (Grant Nos. 11674043 and 11604030), the Fundamental Research Funds for the Central Universities, China (Grant No. 2018CDJDWL0011), the Fundamental and Advanced Research Program of Chongqing, China (Grant No. cstc2018jcyjAX0338), and Arizona State University Start-up Funds, USA. Publisher Copyright: © 2019 Chinese Physical Society and IOP Publishing Ltd.
PY - 2019
Y1 - 2019
N2 - Cancer cell motility and its heterogeneity play an important role in metastasis, which is responsible for death of 90% of cancer patients. Here, in combination with a microfluidic technique, single-cell tracking, and systematic motility analysis, we present a rapid and quantitative approach to judge the motility heterogeneity of breast cancer cells MDA-MB-231 and MCF-7 in a well-defined three-dimensional (3D) microenvironment with controllable conditions. Following this approach, identification of highly mobile active cells in a medium with epithelial growth factor will provide a practical tool for cell invasion and metastasis investigation of multiple cancer cell types, including primary cells. Further, this approach could potentially become a speedy (∼hours) and efficient tool for basic and clinical diagnosis.
AB - Cancer cell motility and its heterogeneity play an important role in metastasis, which is responsible for death of 90% of cancer patients. Here, in combination with a microfluidic technique, single-cell tracking, and systematic motility analysis, we present a rapid and quantitative approach to judge the motility heterogeneity of breast cancer cells MDA-MB-231 and MCF-7 in a well-defined three-dimensional (3D) microenvironment with controllable conditions. Following this approach, identification of highly mobile active cells in a medium with epithelial growth factor will provide a practical tool for cell invasion and metastasis investigation of multiple cancer cell types, including primary cells. Further, this approach could potentially become a speedy (∼hours) and efficient tool for basic and clinical diagnosis.
KW - cancer metastasis
KW - invasion potential
KW - motility heterogeneity
KW - three-dimensional microenvironment
UR - http://www.scopus.com/inward/record.url?scp=85076381601&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85076381601&partnerID=8YFLogxK
U2 - 10.1088/1674-1056/ab3af4
DO - 10.1088/1674-1056/ab3af4
M3 - Article
AN - SCOPUS:85076381601
SN - 1674-1056
VL - 28
JO - Chinese Physics B
JF - Chinese Physics B
IS - 10
M1 - 108701
ER -