Quantitative assessment of multiple pathogen exposure and immune dynamics at scale

Lusheng Song; Femina Rauf; Ching Wen Hou; Ji Qiu; Vel Murugan; Yunro Chung; Huafang Lai; Deborah Adam; D. Mitchell Magee; Guillermo Trivino Soto; Milene Peterson; Karen S. Anderson; Stephen G. Rice; Benjamin Readhead; Jin G. Park; Joshua LaBaer

doi:10.1128/spectrum.02399-23

Quantitative assessment of multiple pathogen exposure and immune dynamics at scale

Lusheng Song, Femina Rauf, Ching Wen Hou, Ji Qiu, Vel Murugan, Yunro Chung, Huafang Lai, Deborah Adam, D. Mitchell Magee, Guillermo Trivino Soto, Milene Peterson, Karen S. Anderson, Stephen G. Rice, Benjamin Readhead, Jin G. Park, Joshua LaBaer

Research output: Contribution to journal › Article › peer-review

Abstract

IMPORTANCE: Serology reveals exposure to pathogens, as well as the state of autoimmune and other clinical conditions. It is used to evaluate individuals and their histories and as a public health tool to track epidemics. Employing a variety of formats, studies nearly always perform serology by testing response to only one or a few antigens. However, clinical outcomes of new infections also depend on which previous infections may have occurred. We developed a high-throughput serology method that evaluates responses to hundreds of antigens simultaneously. It can be used to evaluate thousands of samples at a time and provide a quantitative readout. This tool will enable doctors to monitor which pathogens an individual has been exposed to and how that changes in the future. Moreover, public health officials could track populations and look for infectious trends among large populations. Testing many potential antigens at a time may also aid in vaccine development.

Original language	English (US)
Pages (from-to)	e0239923
Journal	Microbiology Spectrum
Volume	12
Issue number	1
DOIs	https://doi.org/10.1128/spectrum.02399-23
State	Published - Jan 11 2024

Keywords

human microbiome
immunoassays
immunoserology
infectious disease surveillance
molecular diagnosis
multiplex assay
proteomics
virology

ASJC Scopus subject areas

Physiology
Ecology
General Immunology and Microbiology
Genetics
Microbiology (medical)
Cell Biology
Infectious Diseases

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10.1128/spectrum.02399-23

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Song, L., Rauf, F., Hou, C. W., Qiu, J., Murugan, V., Chung, Y., Lai, H., Adam, D., Magee, D. M., Trivino Soto, G., Peterson, M., Anderson, K. S., Rice, S. G., Readhead, B., Park, J. G., & LaBaer, J. (2024). Quantitative assessment of multiple pathogen exposure and immune dynamics at scale. Microbiology Spectrum, 12(1), e0239923. https://doi.org/10.1128/spectrum.02399-23

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doi = "10.1128/spectrum.02399-23",

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AU - Qiu, Ji

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AU - Chung, Yunro

AU - Lai, Huafang

AU - Adam, Deborah

AU - Magee, D. Mitchell

AU - Trivino Soto, Guillermo

AU - Peterson, Milene

AU - Anderson, Karen S.

AU - Rice, Stephen G.

AU - Readhead, Benjamin

AU - Park, Jin G.

AU - LaBaer, Joshua

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Quantitative assessment of multiple pathogen exposure and immune dynamics at scale

Abstract

Keywords

ASJC Scopus subject areas

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