Protocol for electrical modulation of ERK signaling dynamics in cell lines

Kan Zhu; Liang Guo; Preena Patel; John Albeck; Quan Qing; Wolfgang Losert; Min Zhao

doi:10.1016/j.xpro.2022.101752

Protocol for electrical modulation of ERK signaling dynamics in cell lines

Kan Zhu, Liang Guo, Preena Patel, John Albeck, Quan Qing, Wolfgang Losert, Min Zhao

Physics

Research output: Contribution to journal › Article › peer-review

Abstract

Here, we describe a protocol for modulating the dynamics of the extracellular signal-regulated kinases (ERK) pathway in a customized alternating current (AC) electric field stimulation chamber. We use an ERK translocation reporter that can accurately represent the intracellular ERK activity in real time without chemical agents or gene disruption. ERK activation is assessed by comparing the relative intensity of nuclear fluorescence to cytosolic fluorescence in live-cell conditions. The approach can be applied to other signaling pathways as well. For complete details on the use and execution of this protocol, please refer to Guo et al. (2021).

Original language	English (US)
Article number	101752
Journal	STAR Protocols
Volume	3
Issue number	4
DOIs	https://doi.org/10.1016/j.xpro.2022.101752
State	Published - Dec 16 2022

Keywords

Biotechnology and bioengineering
Cell biology
Cell culture
Microscopy
Signal transduction

ASJC Scopus subject areas

General Neuroscience
General Biochemistry, Genetics and Molecular Biology
General Immunology and Microbiology

Access to Document

10.1016/j.xpro.2022.101752

Cite this

@article{6f819153a7cd4d74be5d0b9cfc4559fe,

title = "Protocol for electrical modulation of ERK signaling dynamics in cell lines",

abstract = "Here, we describe a protocol for modulating the dynamics of the extracellular signal-regulated kinases (ERK) pathway in a customized alternating current (AC) electric field stimulation chamber. We use an ERK translocation reporter that can accurately represent the intracellular ERK activity in real time without chemical agents or gene disruption. ERK activation is assessed by comparing the relative intensity of nuclear fluorescence to cytosolic fluorescence in live-cell conditions. The approach can be applied to other signaling pathways as well. For complete details on the use and execution of this protocol, please refer to Guo et al. (2021).",

keywords = "Biotechnology and bioengineering, Cell biology, Cell culture, Microscopy, Signal transduction",

author = "Kan Zhu and Liang Guo and Preena Patel and John Albeck and Quan Qing and Wolfgang Losert and Min Zhao",

note = "Publisher Copyright: {\textcopyright} 2022 The Author(s)",

year = "2022",

month = dec,

day = "16",

doi = "10.1016/j.xpro.2022.101752",

language = "English (US)",

volume = "3",

journal = "STAR Protocols",

issn = "2666-1667",

publisher = "Cell Press",

number = "4",

}

TY - JOUR

T1 - Protocol for electrical modulation of ERK signaling dynamics in cell lines

AU - Zhu, Kan

AU - Guo, Liang

AU - Patel, Preena

AU - Albeck, John

AU - Qing, Quan

AU - Losert, Wolfgang

AU - Zhao, Min

PY - 2022/12/16

Y1 - 2022/12/16

N2 - Here, we describe a protocol for modulating the dynamics of the extracellular signal-regulated kinases (ERK) pathway in a customized alternating current (AC) electric field stimulation chamber. We use an ERK translocation reporter that can accurately represent the intracellular ERK activity in real time without chemical agents or gene disruption. ERK activation is assessed by comparing the relative intensity of nuclear fluorescence to cytosolic fluorescence in live-cell conditions. The approach can be applied to other signaling pathways as well. For complete details on the use and execution of this protocol, please refer to Guo et al. (2021).

AB - Here, we describe a protocol for modulating the dynamics of the extracellular signal-regulated kinases (ERK) pathway in a customized alternating current (AC) electric field stimulation chamber. We use an ERK translocation reporter that can accurately represent the intracellular ERK activity in real time without chemical agents or gene disruption. ERK activation is assessed by comparing the relative intensity of nuclear fluorescence to cytosolic fluorescence in live-cell conditions. The approach can be applied to other signaling pathways as well. For complete details on the use and execution of this protocol, please refer to Guo et al. (2021).

KW - Biotechnology and bioengineering

KW - Cell biology

KW - Cell culture

KW - Microscopy

KW - Signal transduction

UR - http://www.scopus.com/inward/record.url?scp=85139330503&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85139330503&partnerID=8YFLogxK

U2 - 10.1016/j.xpro.2022.101752

DO - 10.1016/j.xpro.2022.101752

M3 - Article

C2 - 36208452

AN - SCOPUS:85139330503

SN - 2666-1667

VL - 3

JO - STAR Protocols

JF - STAR Protocols

IS - 4

M1 - 101752

ER -

Protocol for electrical modulation of ERK signaling dynamics in cell lines

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this